Penicillin-G acylases
View Patent ↗The present invention provides engineered penicillin G acylase (PGA) enzymes having improved properties, polynucleotides encoding such enzymes, compositions including the enzymes, and methods of using the enzymes.
1. An engineered polynucleotide encoding an engineered penicillin G acylase having a polypeptide sequence that is at least 95% identical to SEQ ID NO: 1220, wherein said penicillin G acylase comprises a leucine residue at position X2 as compared to the sequence of SEQ ID NO: 1220.
2. The engineered polynucleotide encoding the engineered penicillin G acylase of claim 1 , wherein said penicillin G acylase comprises SEQ ID NO: 1194.
3. The engineered polynucleotide encoding the engineered penicillin G acylase of claim 1 , wherein said penicillin G acylase is capable of producing phenyl acetate mono-protected or di-protected insulin.
4. The engineered polynucleotide encoding the engineered penicillin G acylase of claim 1 , wherein said penicillin G acylase is encoded by a polynucleotide sequence that is at least 95% identical to SEQ ID NO:1219.
5. The engineered polynucleotide encoding the engineered penicillin G acylase of claim 1 , wherein said penicillin G acylase comprises SEQ ID NO: 1220.
6. A vector comprising the engineered polynucleotide of claim 1 .
7. A host cell comprising the vector of claim 6 .
8. The host cell of claim 7 , wherein said host cell is a prokaryotic or eukaryotic cell.
9. The host cell of claim 8 , wherein said prokaryotic host cell is E. coli.