Placental tissue component compositions for treatment of skin defects and methods using same
The present disclosure provides methods of treating defects such as wrinkles or scars via a subdermal injection of placental tissue components. It also provides placental tissue compositions maintained within the barrel of a syringe, which may be for use in treating such defects.
1. A method of preparing an injectable placental tissue composition, said method comprising:
a) decellularizing placental disk;
b) dehydrating the placental disk;
c) dehydrating one or more of amniotic membrane, chorionic membrane, and umbilical cord;
d) combining the dehydrated placental disk with the one or more of dehydrated amniotic membrane, chorionic membrane, and umbilical cord; and
e) milling the composition formed in step d) so as to form dehydrated particles.
2. The method of claim 1 , further comprising sifting the dehydrated particles using a sieve having a size from 50-500 μm.
3. The method of claim 2 , further comprising rehydrating the sifted, dehydrated particles so as to form a rehydrated composition.
4. The method of claim 3 , further comprising placing the rehydrated composition in a syringe.
5. The method of claim 1 , wherein the dehydrated particles are about 1-20% dehydrated amniotic membrane and/or chorionic membrane particles, if present, about 1-30% dehydrated umbilical cord particles, if present, and about 50-98% dehydrated, decellularized placental disc particles, by weight.
6. The method of claim 1 , wherein the particles comprise placental disc and amniotic membrane.
7. The method of claim 1 , wherein the particles comprise placental disc and chorionic membrane.
8. The method of claim 1 , wherein the particles comprise placental disc, amniotic membrane, and chorionic membrane.
9. The method of claim 1 , wherein the particles comprise placental disc and umbilical cord.
10. The method of claim 1 , wherein the particles comprise placental disc, amniotic membrane, chorionic membrane, and umbilical cord.
11. The method of claim 1 , wherein the particles have a size from about 50-150 μm.