Apparatus and method for fluorescence polarization detection
An analyzing system includes a detection area with a transparent window past which an analyte moves and emits or transmits light. One or more polarizing elements receive and polarize the light into respective two or more different polarization components. One or more optical detectors receive the respective two or more polarization components and generate respective at least two signals in response. A processor is coupled to the optical detectors and configured to determine a polarization status of the light from the analyte based on the at least two signals.
1. An analyzing system, comprising:
a detection area comprising a transparent window past which an analyte moves and emits or transmits light;
one or more polarizing elements that receive and polarize the light into respective two or more different polarization components;
one or more optical detectors that receive the respective two or more polarization components and generate respective at least two signals in response; and
a processor coupled to the optical detectors and configured to determine a polarization status of the light from the analyte based on the at least two signals.
2. The analyzing system of claim 1 , wherein the light from the analyte produces a moving image that passes by the one or more polarizing elements.
3. The analyzing system of claim 2 , wherein the one or more polarizing elements comprises two or more polarizing elements, and the one or more optical detectors comprises one optical detector that overlaps the two or more polarizing elements.
4. The analyzing system of claim 3 , wherein the two or more polarizers are arranged linearly such that the moving image passes sequentially through the two or more polarizers.
5. The analyzing system of claim 3 , wherein the processor determines the polarization status of the light via a time domain signal of the one optical detector, two or more values of the time domain signal at respective two or more time segments corresponding to the respective two or more polarizing elements.
6. The analyzing system of claim 3 , wherein the one optical detector comprises a silicon photomultiplier.
7. The analyzing system of claim 1 , wherein the processor uses spatial modulation to gain at least one of image information and color co-localization of the image.
8. The analyzing system of claim 7 , wherein the image information comprises at least one of size and shape of the analyte.
9. The analyzing system of claim 1 , wherein the polarization status is determined by the processor at greater than 10 kHz.
10. The analyzing system of claim 1 , wherein determining the polarization status comprises determining a circular polarization component of the light and a linear polarization component of the light.
11. The analyzing system of claim 1 , further comprising a light source that illuminates the analyte causing transmission of the light from the analyte.
12. The analyzing system of claim 1 , wherein the polarization status comprises a fluorescence polarization, and wherein the processor is further configured to use the fluorescence polarization to correct a fluorescence intensity detected by the analyzing system.
13. The analyzing system of claim 1 , wherein the analyte is moved within of a jet-in-air channel or a microfluidic channel.
14. A method comprising:
passing an analyte past a detection area;
receiving light from the analyte at one or more polarizing elements that polarize the light into respective two or more different polarization angle components;
directing the two or more different polarization angle components into one or more optical detectors to generate two or more signals corresponding to the two or more different polarization angle components; and
determining a polarization status of the analyte based on the two or more signals.
15. The method of claim 14 , wherein the light from the analyte produces a moving image that passes by the one or more polarizing elements.
16. The method of claim 15 , wherein the one or more polarizing elements comprises two or more polarizing elements arranged linearly such that the moving image passes sequentially through the two or more polarizers, and the one or more optical detectors comprises one optical detector that overlaps the two or more polarizing elements.
17. The method of claim 16 , wherein the polarization status of the light is determined by a time domain signal of the one optical detector, two or more values of the time domain signal at respective two or more time segments corresponding to the respective two or more polarizing elements.
18. The method of claim 14 , further comprising using spatial modulation to gain at least one of image information and color co-localization of the image.
19. The method of claim 18 , wherein the image information comprises at least one of size and shape of the analyte.
20. The method of claim 14 , wherein determining the polarization status comprises determining a circular polarization component of the light and a linear polarization component of the light.