Collagen and micronized placental tissue compositions and methods of making and using the same
Described herein are compositions of collagen and micronized placental tissue components, methods for producing the same and methods for using the same for wound healing, repairing damaged tendons, cosmetic applications and covering biocompatible materials and/or devices.
1. A composition comprising water, soluble collagen and micronized placental tissue components such that the composition is an integrated admixture having apparent homogeneity, wherein the composition is an aqueous or gelatinous composition which forms a coherent and flexible mass when force and/or shear is applied.
2. The composition of claim 1 , wherein the micronized placental tissue components comprise micronized amnion, micronized chorion, micronized Wharton's jelly, or any combination thereof.
3. The composition of claim 1 , wherein the micronized placental tissue components have a particle size less than 400 μm.
4. The composition of claim 1 , wherein the micronized placental tissue components comprise a micronized tissue graft comprising an amnion layer and a chorion layer, wherein the chorion layer is layered directly over the amnion layer, and further wherein the amnion layer has an exposed basement membrane and an intact fibroblast component.
5. The composition of claim 1 , wherein the micronized placental tissue components comprise a micronized tissue graft comprising an amnion layer and a chorion layer, wherein the chorion layer is layered directly over the amnion layer, and further wherein the amnion layer has an intact epithelial cell layer and an intact fibroblast component.
6. The composition of claim 1 , wherein the collagen is human collagen.
7. The composition of claim 1 , wherein the collagen is an aqueous collagen material comprising from about 0.1% to about 2% collagen.
8. The composition of claim 1 , wherein the weight ratio of the micronized placental tissue components to the collagen is from about 3:1 to about 100:1.
9. The composition of claim 1 , wherein the weight ratio of micronized placental tissue components to collagen is about 100:1 to about 300:1.
10. The composition of claim 1 , wherein the composition is obtained by ejecting a precursor composition comprising aqueous/gelatinous collagen and micronized placental tissue components.
11. A method for transforming an aqueous/gelatinous collagen mass into a coherent and flexible composition said method comprising:
a. incorporating sufficient micronized placental tissue components into an aqueous collagen material; and
b. applying sufficient force and shear to the composition so as to transform the composition into a coherent, flexible mass.
12. The method of claim 11 , wherein the aqueous collagen material comprises from about 0.1% to about 2% collagen.
13. The method of claim 11 , wherein the micronized placental tissue components comprise micronized amnion, micronized chorion, micronized Wharton's jelly, or any combination thereof.
14. The method of claim 11 , wherein the placental tissue components have a particle size less than 400 μm.
15. The method of claim 11 , wherein the collagen is human collagen.
16. The method of claim 11 , wherein the weight ratio of the micronized placental tissue components to the collagen is from about to about 100:1 to about 300:1.
17. A method for treating a wound in a subject in need thereof comprising administering to the wound an effective amount of the composition of claim 1 .
18. A method for treating a cosmetic defect in a subject in need thereof comprising administering to the wound an effective amount of the composition of claim 1 .