Penicillin-G acylases
The present invention provides engineered penicillin G acylase (PGA) enzymes, polynucleotides encoding the enzymes, compositions comprising the enzymes, and methods of using the engineered PGA enzymes.
1. An engineered polynucleotide sequence encoding an engineered penicillin G acylase capable of acylating insulin, wherein the polypeptide sequence of said penicillin G acylase is at least 98%, 99% or more identical to SEQ ID NO:100.
2. The engineered polynucleotide sequence of claim 1 , wherein said sequence comprises a polynucleotide sequence that is at least 98%, 99% or more identical to SEQ ID NO: 99.
3. A vector comprising the polynucleotide sequence of claim 2 .
4. The vector of claim 3 , further comprising at least one control sequence.
5. A host cell comprising the vector of claim 3 .