IP Library Granted Patent US 12,577,179
Granted Patent B2
US 12,577,179 · App. 18/012,066 · Granted Mar 17, 2026

Methods and compositions for soil regeneration and improved soil hydrology

Inventors: Sean Farmer (Ft. Lauderdale, FL); Karthik N. Karathur (Solon, OH)
Assignee: LOCUS SOLUTIONS IPCO, LLC
C05F11/08C05G3/50C09K17/14B82Y5/00
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Quick Facts
Patent No.
US 12,577,179
App. No.
18/012,066
Filed
Dec 21, 2022
Granted
Mar 17, 2026
Kind
B2
Art Unit
1736
USPC
71/6
Abstract

The subject invention provides compositions and methods of their use for improving soil structure and hydrology. Advantageously, the compositions and methods of the subject invention can be formulated as environmentally-friendly, non-toxic and cost-effective solutions to the growing problems of, for example, water shortages, water-use inefficiency, declining soil health, nutrient leaching and runoff, and soil-borne greenhouse gas emissions.

Claims (15)

1 . A method of irrigating soil and/or improving one or more properties of soil, which comprises applying an irrigation additive to soil, wherein said irrigation additive comprises a biosurfactant, and wherein said irrigation additive lowers the surface tension of an aqueous fluid applied to the soil, thereby improving dispersion, penetration and/or percolation of the aqueous fluid throughout the soil, and

applying a microbial soil treatment composition to the soil about 30 days after application of the irrigation additive, wherein the microbial soil treatment composition comprises one or more beneficial microorganisms capable of colonizing soil and/or plant roots.

2 . The method of claim 1 , wherein the biosurfactant a microbial derived biosurfactant, or a surfactant derived from a naturally-occurring substrate, and wherein the surface active molecule has a micelle size less than 25 nm.

3 . The method of claim 1 , wherein the biosurfactant is selected from glycolipids, lipopeptides, flavolipids, phospholipids, fatty acid esters, lipoproteins, lipopolysaccharide-protein complexes, and polysaccharide-protein- fatty acid complexes.

4 . The method of claim 1 , wherein the biosurfactant is a sophorolipid.

5 . The method of claim 1 , wherein the irrigation additive is mixed with the aqueous liquid and applied to the soil via an irrigation system.

6 . The method of claim 1 , wherein the irrigation additive is applied to soil before or after the soil is irrigated with an aqueous liquid.

7 . The method of claim 1 , wherein the irrigation additive is applied at a concentration of 10 to 100 ppm with respect to the amount of aqueous fluid.

8 . The method of claim 1 , wherein the property of soil being improved is soil compaction, and wherein the irrigation additive improves compaction of the soil by increasing porosity of the soil.

9 . The method of claim 1 , wherein the irrigation additive is applied again about 30 days after application of the microbial soil treatment composition, followed by re-application of the microbial soil treatment composition after 30 more days, and wherein this is repeated until a desired level of irrigation and/or improvement in the one or more properties of soil is achieved.

10 . The method of claim 1 , wherein at least one of the one or more beneficial microorganisms is selected from Trichoderma harzianum, Trichoderma viride, Trichoderma koningii, Trichoderma guizhouse, Bacillus amyloliquefaciens, Bacillus subtilis, Bacillus megaterium, Bacillus polymyxa, Bacillus licheniformis, Brevibacillus laterosporus, Meyerozyma guilliermondii, Pichia occidentalis, Pichia kudriavzevii, Wickerhamomyces anomalus , and Debaryomyces hansenii.

11 . The method of claim 1 , comprising applying a beneficial microorganism selected from Bacillus amyloliquefaciens NRRL B-67928 and a Trichoderma harzianum.

12 . The method of claim 1 , comprising applying a beneficial microorganism that is Wickerhamomyces anomalus NRRL Y-68030.

13 . The method of claim 1 , comprising applying a beneficial microorganism that is B. subtilis B4 NRRL B-68031.

14 . The method of claim 1 , wherein water usage is reduced by at least 25% compared with soils irrigated without the irrigation additive.

Assignments (5)
SECURITY INTEREST Recorded Nov 11, 2024
From: LOCUS SOLUTIONS IPCO, LLC
To: U.S. BANK TRUST COMPANY, NATIONAL ASSOCIATION, AS COLLATERAL AGENT
Reel/Frame 069333/0911 →
RELEASE OF SECURITY INTEREST Recorded Oct 30, 2024
From: U.S. BANK TRUST COMPANY, NATIONAL ASSOCIATION, AS COLLATERAL AGENT
To: LOCUS SOLUTIONS IPCO, LLC
Reel/Frame 069273/0361 →
SECURITY INTEREST Recorded Dec 8, 2023
From: LOCUS SOLUTIONS IPCO, LLC
To: U.S. BANK TRUST COMPANY, NATIONAL ASSOCIATION, AS THE COLLATERAL AGENT
Reel/Frame 065836/0868 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jan 6, 2023
From: FARMER, SEAN; KARATHUR, KARTHIK N.
To: LOCUS IP COMPANY, LLC
Reel/Frame 062293/0099 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jan 6, 2023
From: LOCUS IP COMPANY, LLC
To: LOCUS SOLUTIONS IPCO, LLC
Reel/Frame 062309/0215 →
Continuity (2)
Provisional Application 63256688 · Oct 18, 2021
Related Publication 20240116831A1 · Apr 11, 2024
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