Combinations of imaging agent conjugates and application thereof
The present invention provides a combination of two or more imaging agent conjugates comprising an imaging agent conjugated to a targeting ligand, wherein the imaging agent comprises one or more of ZW800-1, ZW830-1, ZW700-1-Forte, ZW-DOTA, ZW-PyC3A, ZW-Macropa, ZW-porphyrin, ZW-NOTA, and ZW-Deferoxamine; and the targeting ligand comprises one or more of a cRGD, a dPSMA-617 or a KUE, a FAP, a bombesin receptor binding vector, or a octreotide binding vector, or one or more of peptides selected from SEQ ID No: 1-4.
1 . A composition comprising a combination of imaging agents which absorb invisible near-infrared fluorescent light at the same wavelength; wherein the composition comprises a combination of:
a) cRGD-ZW800-1, KUE-ZW800-1, and FAP-ZW800-1;
b) cRGD-ZW700-1 Forte, KUE-ZW700-1 Forte, and FAP-ZW700-1 Forte; or
c) cRGD-ZW830-1, KUE-ZW830-1, and FAP-ZW830-1.
2 . A method of imaging cells, the method comprising:
a) contacting cells with a composition according to claim 1 ,
b) irradiating the cells at a wavelength absorbed by the combination of imaging agents; and
c) and detecting a signal from the combination of imaging agents, thereby imaging the cells.
3 . The method of claim 2 , wherein the cells are tumor cells, inflammatory cells, or cells undergoing angiogenesis.
4 . The method of claim 2 , wherein the combination of imaging agents is administered to an organism comprising or suspected of comprising the cells.
5 . The method of claim 4 , wherein the organism is human.
6 . The method of claim 2 , wherein the cells are is imaged in vivo.