Multifunctional, hydrogel hybrid material, the method of its preparation and the use in the treatment of bone losses

1 . A method of producing a multifunctional hydrogel hybrid material which contains a biopolymer matrix containing collagen, chitosan, and lysine-modified hyaluronic acid, silica-apatite particles functionalized with amino groups, an active substance in the form of alendronate attached to silica-apatite particles, and genipin as a cross-linking substance, the method comprising the following steps:

a) functionalizing silica particles with amino groups,

b) suspending the silica particles obtained in step a) in an aqueous SBF solution to obtain, after 10 days of incubation, silica-apatite particles,

c) attaching sodium alendronate to the silica-apatite particles obtained in step b),

d) adding a solution of the collagen, the chitosan and the lysine-modified hyaluronic acid to an aqueous suspension of the particles obtained in step c), and

e) subjecting the mixture obtained in step d) to a cross-linking reaction with genipin,

thereby producing the multifunctional hydrogel hybrid material.

2 . The method according to claim 1 , wherein functionalizing said silica particles with amino groups is carried out by the sol-gel method.

3 . A multifunctional hydrogel hybrid material obtained by a method as defined in claim 1 .

4 . A multifunctional hydrogel hybrid material obtained by a method as defined in claim 2 .

5 . A method of treating bone losses in a subject in need thereof, the method comprising administering to the subject a therapeutically effective amount of the multifunctional hydrogel hybrid material as defined in claim 3 .

6 . The method according to claim 5 , wherein the bone losses are due to osteoporosis.

7 . The method according to claim 1 , wherein suspending the silica particles obtained in step a) in an aqueous SBF solution is at a concentration of 1.5 M.