Diagnostic device with integrated sampler and holder

1 . An analytical device comprising

A) A sample region comprising a first opening and a first cavity within the device and closure means for covering the first opening, wherein the first opening and first cavity is configured to receive a portion of a sampler,

B) A second cavity comprising an extraction solvent or extraction reagent within the device, wherein at least a portion of the second cavity is above the sample region,

C) An extraction region configured to receive at least a portion of the sampler from the sample region and at least a portion of the reaction extraction solvent or extraction reagent, wherein the extraction region comprises a first extraction region and a second extraction region separated by a wall or partition,

D) A reaction region comprising one or more reaction reagents wherein the reaction region is located downstream of the extraction region and is configured to receive liquid flowing from the extraction region,

E) A first fluid passage connecting the extraction region to the reaction region wherein the first fluid passage comprises a first surface energy gradient coating,

F) A detection region comprising one or more detection agents wherein the detection region is located downstream of the reaction region and is configured to receive liquid flowing from the reaction region.

2 . The analytical device of claim 1 , wherein the first surface energy gradient coating has a formula X-J-M;

where X forms a bond to the surface and is selected from the group comprising: a carboxylic acid, a sulfur-containing functional group, silanes, chlorosilanes, and hydroxamic acid;

J is a spacer moiety or polymer backbone and is a hydrocarbon chain, (CH2)n, where n is between 1 and 22;

M is a functional group and is selected from the group comprising ionic, nonionic, polar, nonpolar, halogenated, alkyl, aryl, OH, CONHR, CONHCOR, NHR, COOH, COOR, CSNHR, COR, RCSR, RSR, ROR, SOOR, RSOR, CONR2, (OCH2 CH2)nOH, CH3, NR2, CN, (CF2)nCF3, CO2CH3, CONHCH3, CR, CHCH2, OCH2CF2CF3, Cl, Br, olefins, and combination thereof.

3 . The analytical device of claim 1 , wherein the extraction solvent comprises a phosphate buffer solution with a pH of 7 to 9.

4 . The analytical device of claim 1 , wherein the detection region comprises at least a portion of a lateral flow strip.

5 . The analytical device of claim 1 , wherein the reaction region comprises at least a portion of a lateral flow strip.

6 . The analytical device of claim 1 , further comprising a second fluid passage connecting the reaction region to the detection region wherein the second fluid passage comprises a second surface energy gradient coating.

7 . The analytical device of claim 6 , wherein the second surface energy gradient coating has a formula X-J-M;

where X forms a bond to the surface and is selected from the group comprising: a carboxylic acid, a sulfur-containing functional group, silanes, chlorosilanes, and hydroxamic acid;

J is a spacer moiety or polymer backbone and is a hydrocarbon chain, (CH2)n, where n is between 1 and 22;

M is a functional group and is selected from the group comprising ionic, nonionic, polar, nonpolar, halogenated, alkyl, aryl, OH, CONHR, CONHCOR, NHR, COOH, COOR, CSNHR, COR, RCSR, RSR, ROR, SOOR, RSOR, CONR2, (OCH2 CH2)nOH, CH3, NR2, CN, (CF2)nCF3, CO2CH3, CONHCH3, CR, CHCH2, OCH2CF2CF3, Cl, Br, olefins, and combination thereof.

8 . The analytical device of claim 1 , wherein the wall or partition separating the first extraction region from the second reaction region has a height shorter than the height of the extraction region.

9 . The analytical device of claim 1 , wherein the second cavity comprises an opening with a seal.

10 . The analytical device of claim 1 , wherein the second cavity comprises a pierceable container.