Nucleic acid extraction device
Provided herein is a nucleic acid extraction and the structure of a reaction chamber is improved such that a piston, a reaction chamber and a magnetic rod are independent of one another, magnetic beads are more concentrated, more sufficient adsorption, washing and elution are achieved, dead corners are eliminated, when the nucleic acid extraction and amplification device is used to perform nucleic acid extraction and amplification. The sensitivity and accuracy of nucleic acid detection can be further improved, and missing and wrong detection can be avoided.
1 . A nucleic acid extraction device, comprising:
a driving component, a piston, a turntable, a plurality of chambers, a permanent magnet, and a plurality of magnetic beads;
wherein the driving component is configured to carry and drive components of the nucleic acid extraction device to work, the driving component comprises a piston driving mechanism and a worm gear and worm transmission mechanism, the worm gear and worm transmission mechanism comprises a worm, a worm shaft, a worm gear, an elastic element, and a connecting module;
the piston driving mechanism is configured to drive the piston of the nucleic acid extraction device to move vertically;
the worm gear and worm transmission mechanism is configured to drive the turntable to rotate, thereby connecting or disconnecting the plurality of chambers of the nucleic acid extraction device; and the worm gear and worm transmission mechanism is provided with the elastic element capable of resetting, which is configured to provide thrust to the worm shaft to make the worm close to the worm gear so as to eliminate a backlash;
wherein the elastic element capable of resetting is a reset spring, the reset spring is located at a first end of the worm shaft, and a second end of the worm shaft is flexibly fixed by means of an angular contact bearing;
wherein a first end of the reset spring is movably connected to the worm shaft by means of the connecting module, and the connecting module is provided with an arc-shaped recess and is in direct contact with the worm shaft by means of the arc-shaped recess;
wherein the plurality of chambers comprises a reaction chamber, the piston is configured to control a flow direction of a fluid; the reaction chamber is configured to provide a place for the magnetic beads to adsorb, wash and elute nucleic acids; the permanent magnet is configured to provide a magnetic attraction force for the magnetic beads; the permanent magnet is a magnetic rod; and the piston is located inside or outside the reaction chamber.
2 . The nucleic acid extraction device according to claim 1 , wherein the connecting module is made of lubricating materials, and the arc-shaped recess is provided with an arc-shaped rib which matches with an arc-shaped groove in the worm shaft.
3 . The nucleic acid extraction device according to claim 1 , wherein the connecting module is made of POM or Teflon materials, a second end of the reset spring is fixed to a base, and the reset spring has an elastic modulus of 0.5-1.0 N/m2.
4 . A nucleic acid extraction device, comprising:
a driving component, a piston, a turntable, a plurality of chambers, a permanent magnet, and a plurality of magnetic beads;
wherein the driving component is configured to carry and drive components of the nucleic acid extraction device to work, the driving component comprises a piston driving mechanism and a worm gear and worm transmission mechanism, the worm gear and worm transmission mechanism comprises a worm, a worm shaft, a worm gear, an elastic element, and a connecting module;
the piston driving mechanism is configured to drive the piston of the nucleic acid extraction device to move vertically;
the worm gear and worm transmission mechanism is configured to drive the turntable to rotate, thereby connecting or disconnecting the plurality of chambers of the nucleic acid extraction device; and the worm gear and worm transmission mechanism is provided with the elastic element capable of resetting, which is configured to provide thrust to the worm shaft to make the worm close to the worm gear so as to eliminate a backlash,
wherein the plurality of chambers comprises a reaction chamber, the piston is configured to control a flow direction of a fluid; the reaction chamber is configured to provide a place for the magnetic beads to adsorb, wash and elute nucleic acids; the permanent magnet is configured to provide a magnetic attraction force for the magnetic beads; the permanent magnet is a magnetic rod; and the piston is located inside or outside the reaction chamber;
wherein when the piston is located inside the reaction chamber, the reaction chamber is internally provided with the piston and a hollow accommodating chamber; the hollow accommodating chamber is configured to accommodate the permanent magnet, the piston is a hollow cylinder, and the hollow cylinder in the piston has the diameter matching that of the hollow accommodating chamber, so that the piston can be sleeved outside the hollow accommodating chamber and move vertically along an outer wall of the hollow accommodating chamber; and the displacement of the piston causes the pressure change, thereby promoting the fluids to flow between the reaction chamber and other chambers.
5 . The nucleic acid extraction device according to claim 4 , wherein the piston driving mechanism comprises a combined movement component and a separated movement component; the combined movement component can combine the piston with a piston rod, thereby enabling the piston rod to drive the piston to move vertically; and the separated movement component prevents the piston from moving by ejecting a push rod, thereby enabling the piston to be separated from the piston rod that moves continuously; and the push rod is controlled by an electromagnet to be ejected or retracted, the push rod is ejected when the electromagnet is energized in one direction, and the push rod is retracted when the electromagnet is energized in the opposite direction.
6 . The nucleic acid extraction device according to claim 5 , wherein the combined movement component comprises a movement module, the movement module is configured to control the vertical movements of the piston and the piston rod, and a jacking plate is arranged below the movement module and can move vertically with the movement module; the piston rod penetrates through the jacking plate to be integrally connected with the movement module, and the jacking plate is movably sleeved on the piston rod from bottom to top; a spring is arranged below the jacking plate, when the push rod is ejected to prevent the jacking plate from moving upwards, the spring is compressed, and when the push rod is retracted, the spring is restored to push the jacking plate to return upwards; and the spring is sleeved on a slide rod, the slide rod penetrates through the jacking plate to be connected to the movement module, the jacking plate can move vertically along the slide rod, a base is arranged below the slide rod, and the base has a diameter larger than that of the spring.
7 . The nucleic acid extraction device according to claim 6 , wherein the combined movement component comprises a groove formed in an outer wall of the piston rod and a rib arranged on an inner wall of the piston; when the piston rod moves downwards to be inserted into the piston, the groove can match with the rib, so that the piston and the piston rod are combined; the piston is the hollow cylinder, and an upper end of the piston has a cross section diameter larger than that of a lower end and matching that of the piston rod, so that the piston rod and the upper end of the piston can be closely combined; and a cylindrical wall of the upper end of the piston is provided with a longitudinal open groove, when the piston rod is inserted into the open groove, the cross section diameter of the upper end of the piston can be increased under the effect of the open groove, and thus the piston rod can be conveniently inserted into the piston and combined with the rib by means of the groove.
8 . The nucleic acid extraction device, comprising:
a driving component, a piston, a turntable, a plurality of chambers, a permanent magnet, and a plurality of magnetic beads;
wherein the driving component is configured to carry and drive components of the nucleic acid extraction device to work, the driving component comprises a piston driving mechanism and a worm gear and worm transmission mechanism, the worm gear and worm transmission mechanism comprises a worm, a worm shaft, a worm gear, an elastic element, and a connecting module;
the piston driving mechanism is configured to drive the piston of the nucleic acid extraction device to move vertically;
the worm gear and worm transmission mechanism is configured to drive the turntable to rotate, thereby connecting or disconnecting the plurality of chambers of the nucleic acid extraction device; and the worm gear and worm transmission mechanism is provided with the elastic element capable of resetting, which is configured to provide thrust to the worm shaft to make the worm close to the worm gear so as to eliminate a backlash,
wherein the plurality of chambers comprises a reaction chamber, the piston is configured to control a flow direction of a fluid; the reaction chamber is configured to provide a place for the magnetic beads to adsorb, wash and elute nucleic acids; the permanent magnet is configured to provide a magnetic attraction force for the magnetic beads; the permanent magnet is a magnetic rod; and the piston is located inside or outside the reaction chamber;
wherein when the piston is located outside the reaction chamber, the permanent magnet and the piston are separately arranged; the piston is located above the reaction chamber; the permanent magnet is located below the reaction chamber; and the permanent magnet is the vertically placed magnetic rod, and the piston, the reaction chamber and the magnetic rod are arranged vertically in sequence from top to bottom.
9 . The nucleic acid extraction device according to claim 8 , wherein the reaction chamber is a recess with an upward opening that is provided in the center of the channel turntable; the piston moves vertically in a piston channel, and the piston channel is in communication with the reaction chamber; and a micro-channel in communication with the reaction chamber is provided in the channel turntable.
10 . The nucleic acid extraction device according to claim 9 , wherein the size of the lower end of the piston and the size of a lower end of the piston channel are reduced; when the piston moves downwards to the lowest position, the piston is blocked by the piston channel and cannot enter the reaction chamber; and when the piston moves downwards to the lowest position, a bottom surface of the piston and the reaction chamber in the center of the channel turntable form a sealed chamber jointly.
11 . The nucleic acid extraction device according to claim 10 , wherein a lower portion of the channel turntable is provided with a cross-shaped fixing element, a central rotating shaft of the fixing element is a hollow cylinder, and the hollow cylinder is a magnetic rod channel for the vertical displacement of the magnetic rod; a bottom surface of the reaction chamber is a top end of the magnetic rod channel; the lower end of the magnetic rod is provided with a magnetic rod driving mechanism for controlling the vertical displacement of the magnetic rod in the magnetic rod channel; and the upper end of the piston is provided with the piston driving mechanism for controlling the vertical displacement of the piston in the piston channel.
12 . The nucleic acid extraction device according to claim 11 , wherein the piston driving mechanism comprises a vertical movement module and a lateral movement module; the vertical movement module is configured to drive the piston to move vertically; the lateral movement module is configured to realize the combination or separation of the piston and the piston driving mechanism; and the vertical movement module and the lateral movement module are integrally nested and combined.
13 . The nucleic acid extraction device according to claim 12 , wherein the lateral movement module comprises a curved rod structure and a slider structure; the curved rod structure is located above the slider structure; the curved rod structure rotates to enable the slider structure to move left and right, so as to drive the piston and the driving mechanism to be combined or separated; the curved rod structure comprises a fixed shaft and a rocking handle, one end of the rocking handle is connected to the fixed shaft, and the rocking handle can perform the circular movement around the fixed shaft; the slider structure is provided with a slide rail with an upward opening; the other end of the rocking handle is provided with a connecting rod; and in the process of the circular movement of the rocking handle, when the connecting rod is inserted into the slide rail, the rocking handle rotates to drive, by means of the connecting rod, the slide rail to move, thereby driving the slider structure to move left and right.
14 . The nucleic acid extraction device according to claim 13 , wherein a bottom surface of the slider structure is provided with an arc-shaped notch with an opening; the piston comprises a top cover and the piston rod, and the top cover has the diameter greater than the cross section diameter of the piston rod; when the slider structure gets close to the piston, an open portion of the arc-shaped notch is sleeved on the piston rod, so that the piston is combined with the driving mechanism and moves vertically with the vertical movement module; and the lateral movement module is integrally nested with the vertical movement module by means of the slider structure and can move left and right relative to the vertical movement module.
15 . The nucleic acid extraction device according to claim 14 , wherein the slider structure is provided with two bumps, which are respectively a first bump and a second bump; the vertical movement module is provided with two grooves, which are respectively a first groove and a second groove; when the vertical movement module and the lateral movement module are integrally nested and combined, the first bump and the second bump are embedded into the first groove and the second groove respectively; and when the lateral movement module moves left and right, the first bump and the second bump slide left and right in the first groove and the second groove respectively.
16 . The nucleic acid extraction device according to claim 15 , wherein the nucleic acid extraction and amplification component comprises a nucleic acid extraction assembly and a nucleic acid amplification assembly; the nucleic acid extraction assembly is configured to extract the nucleic acids in samples; the piston, the reaction chamber and the permanent magnet belong to the nucleic acid extraction assembly; the nucleic acid amplification assembly is configured to amplify the nucleic acids extracted by the nucleic acid extraction assembly; the nucleic acid extraction assembly further comprises a pyrolysis bin, a washing bin and an elution bin; the pyrolysis bin is configured to store the samples, a pyrolysis liquid, the magnetic beads and a waste liquid generated after pyrolysis; the washing bin is configured to store a washing liquid and a waste liquid generated after washing; one or more washing bins are provided; the elution bin is configured to store an eluent; through holes are provided in a bottom of the pyrolysis bin, a bottom of the washing bin and a bottom of the elution bin; the micro-channel is provided in the channel turntable; and the channel turntable can be rotated to enable the micro-channel to be in selective communication with the bottom through hole of the pyrolysis bin, the washing bin or the elution bin, so that the reaction chamber is in selective communication with the pyrolysis bin, the washing bin or the elution bin.
17 . The nucleic acid extraction device according to claim 16 , wherein the nucleic acid amplification assembly comprises a PCR tube and a PCR tube channel; after the nucleic acid extraction is completed, the channel turntable is rotated to enable the micro-channel to be in selective communication with the PCR tube channel, so that the reaction chamber is in selective communication with the PCR tube; the PCR tube is fixedly or detachably connected to a bottom surface of the nucleic acid extraction and amplification device, and the sealed connection can be realized after the connection; and the PCR tube channel at an inlet of the PCR tube deviates from the center line of the PCR tube.