Method for promoting and improving properties of adipose tissue , tissue and cells obtained by said method

1 . A method for promoting anti-inflammatory properties and for improving repair ability of a tissue containing mesenchymal stem cells (MSCs), comprising:

mechanically activating an adipose tissue by applying an orbital shaking force on the adipose tissue that has been lipoaspirated or removed from a body,

wherein the orbital shaking force has an intensity ranging from 40 to 120×g and is applied for a time ranging from 3 to 20 minutes, thereby obtaining an activated adipose tissue having improved anti-inflammatory properties and improved repair ability.

2 . The method according to claim 1 , wherein the applied orbital shaking force has an intensity equal to 97×g.

3 . The method according to claim 1 , wherein said orbital shaking force is applied for 10 minutes.

4 . The method according to claim 1 , wherein mechanically activating comprises applying the orbital shaking force that is repeated two or more times with an equal or different force intensity each time, or with an equal or different force application duration for each time.

5 . The method according to claim 1 , wherein said lipoaspirated adipose tissue is cryopreserved.

6 . The method according to claim 1 , further comprising a step of isolating and expanding the mesenchymal stem cells contained in said adipose tissue that has been lipoaspirated or removed from the body.

7 . The method according to claim 6 , wherein the step of isolating and expanding the mesenchymal stem cells contained in said adipose tissue that has been lipoaspirated or removed from the body comprises:

inoculating 2 ml of the activated adipose tissue in a 25 cm 2 flask completely filled with culture medium “Dulbecco's modified Eagle's Medium” (DMEM) supplemented with 20% fetal bovine serum;

incubating at 37° C. in 5% CO 2 ;

turning upside-down the flask such that cells float up and adhere to a top inner ceiling surface of the flask;

removing the culture medium and tissue after 15 days and turning upside-down the flask so that the cells are on a bottom of the flask; and

replacing the culture medium every 4 days until reaching cell confluence of 90-95%.