Production of fatty acid derivatives
The disclosure relates to recombinant host cells including strain modifications effective to improve titer, yield and/or productivity of fatty acid derivatives. The disclosure further relates to cell cultures including the recombinant host cells for the fermentative production of fatty acid derivatives and compositions thereof.
1 . A recombinant bacterial host cell expressing a polypeptide having 3-hydroxy-acyl-[acp] dehydratase activity;
wherein the polypeptide having 3-hydroxy-acyl-[acp] dehydratase activity is FabA;
wherein the recombinant bacterial host cell expresses one or more additional polypeptides, and the one or more additional polypeptides are FabV, FabH, FabD, FabG, and FabF; and
wherein the recombinant bacterial host cell produces a fatty acid derivative composition at a higher titer, yield, or productivity than a corresponding wild-type host cell when cultured in a medium containing a carbon source.
2 . The recombinant bacterial host cell of claim 1 ,
wherein the polypeptide with 3-hydroxy-acyl-[acp] dehydratase activity is FabA from Salmonella typhimurium ; and
wherein the one or more additional polypeptides are FabV from Vibrio cholerae ; FabH from Salmonella typhimurium, Acinetobacter sp. ADP1, Marinobacter aquaeoli VT8, or Rhodococcus opacus B4; FabD from Salmonella typhimurium ; FabG from Salmonella typhimurium ; and FabF from Clostridium acetobutylicum.
3 . The recombinant bacterial host cell of claim 1 , wherein the polypeptide with 3-hydroxy-acyl-[acp] dehydratase activity and the one or more additional polypeptides are (1) heterologous, or (2) are native and are overexpressed.
4 . The recombinant bacterial host cell of claim 1 , further comprising a genetically engineered polynucleotide sequence encoding an acyl carrier protein (ACP), wherein the recombinant bacterial host cell produces a fatty acid derivative composition at a higher titer, yield, or productivity than a corresponding wild type host cell when cultured in a medium containing a carbon source under conditions effective to express said ACP.
5 . The recombinant bacterial host cell of claim 4 , wherein the ACP is derived from the same organism as a terminal pathway enzyme expressed in the recombinant bacterial host cell, and wherein the terminal pathway enzyme cleaves any acyl-ACP species that is part of a fatty acid biosynthetic pathway.
6 . The recombinant bacterial host cell of claim 1 , wherein the recombinant bacterial host cell further comprises a recombinant polynucleotide encoding a polypeptide that has phosphopantetheinyl transferase activity.
7 . The recombinant bacterial host cell of claim 6 , wherein the polypeptide that has phosphopantetheinyl transferase activity is encoded by an sfp gene.
8 . The recombinant bacterial host cell of claim 1 , wherein the recombinant bacterial host cell further comprises a recombinant polynucleotide encoding a polypeptide that has acetyl-CoA carboxylase activity.
9 . The recombinant bacterial host cell of claim 8 , further comprising a recombinant polynucleotide encoding a polypeptide that has phosphopantetheinyl transferase activity.