IP Library Granted Patent US 12662689
Granted Patent B2
US 12662689 · App. 17/639,765 · Granted Jun 23, 2026

Promoter and method for producing desired substance using same

Inventors: Byoung Hoon Yoon (Seoul, KR); Jin Sook Chang (Seoul, KR); Seon Hye Kim (Seoul, KR); Ji Hye Lee (Seoul, KR); Sun Hyoung Choi (Seoul, KR); Kyungrim Kim (Seoul, KR); Hyung Joon Kim (Seoul, KR)
Assignee: CJ CHEILJEDANG CORPORATION
C12P13/06C12N1/205C12N9/0006C12N15/77C12Y101/01086C12N2800/101C12R2001/15
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Quick Facts
Patent No.
US 12662689
App. No.
17/639,765
Granted
Jun 23, 2026
Kind
B2
Abstract

The present application relates to a novel promoter and a method for producing a desired substance using the same.

Claims (19)

1 . A polynucleotide comprising a nucleotide sequence represented by General Formula 1 below:

X-Y-Z  [General Formula 1]

wherein,

X is CN 1 GN 2 ,

Y is CTAATTN 3 , and

Z is CATGTGTGTGGTATAAT,

in which N 1 of X is cytosine (C) or guanine (G), N 2 of X is adenine (A) or thymine (T), and N 3 of Y is adenine (A) or guanine (G).

2 . The polynucleotide of claim 1 , wherein X is any one of SEQ ID NOS: 8 to 11, and Y is SEQ ID NO: 6 or 7.

3 . The polynucleotide of claim 1 , wherein the polynucleotide comprises any one polynucleotide sequence selected from SEQ ID NOS: 13 to 20.

4 . A promoter comprising the polynucleotide of claim 1 .

5 . A vector comprising the promoter of claim 4 and a gene encoding a target protein.

6 . The vector of claim 5 , wherein the target protein is acetohydroxy acid isomeroreductase.

7 . A microorganism of the genus Corynebacterium , comprising the polynucleotide of claim 1 .

8 . The microorganism of claim 7 , wherein the microorganism of the genus Corynebacterium is Corynebacterium glutamicum.

9 . A method for producing a target substance, the method comprising:

culturing the microorganism of the genus Corynebacterium of claim 7 in a medium; and

recovering the target substance in the medium.

10 . The method of claim 9 , wherein the target substance is an amino acid.

11 . The method of claim 10 , wherein the amino acid is an L-branched-chain amino acid.