Non-invasive gene mutation detection in lung cancer patients
View Patent ↗A system and method for the detection of saliva biomarkers in bodily fluids is described. In particular, the system is suitable for detecting biomarkers of lung cancer in a subject. The system includes an electrochemical sensor chip having at least one well, wherein the at least one well contains a working electrode coated with a conducting polymer functionalized with at least one capture probe, and at least one labeled detector probe. When the at least one labeled detector probe is mixed with a sample of the subject containing a biomarker of lung cancer and added to the at least one well, an electric current is applied to the sample, such that when at least some of the biomarker binds to the capture probe, a measurable change in electric current in the sample is created that is indicative of lung cancer.
1 . A method of detecting lung cancer in a subject comprising:
obtaining a sample from the subject;
mixing a first portion of the sample with a solution comprising a labeled detector probe;
adding the mixture to a first electrode chip on a device, the electrode chip comprising a working electrode, a counter electrode, and a reference electrode; wherein the working electrode is coated with a conducting polymer embedded with a capture probe capable of binding to a marker associated with lung cancer in the sample;
generating 5 cycles to about 10 cycles of a cyclic square wave electric fields field across the electrode for each surface reaction; and
measuring the current in the electrode chip, wherein a change in current is correlated to the presence of the marker associated with lung cancer in the sample
wherein the capture and detection probes are selected from the group consisting of
i) the capture probe comprises the nucleotide sequence of SEQ ID NO: 1 and the detector probe comprises the nucleotide sequence of SEQ ID NO: 2; and
ii) the capture probe comprises the nucleotide sequence of SEQ ID NO: 3 and the detector probe comprises the nucleotide sequence of SEQ ID NO: 4.
2 . The method of claim 1 , wherein the sample is selected from the group consisting of a saliva sample and a blood sample.
3 . The method of claim 1 , wherein the change in current in the sample is measurable within 10 minutes after the sample has been loaded into the well.