Methods and user interfaces for designing cell-based screening assay experiments
A method for designing a cell-based screening assay experiment includes, at a graphical user interface, receiving a first input identifying a template plate definition and receiving a second input defining a first group of disease reagents and at least one destination concentration of the disease reagents. The method also includes receiving a third input defining a first group of treatment reagents, at least one destination concentration for the treatment reagents, a number of replicates of the treatment reagents, and a selection to associate the first group of treatment reagents with the first group of disease reagents. The method further includes generating, based at least partially on the inputs, a design for the cell-based screening assay experiment, including determining a number of test wells required for performing the experiment, and distributing the test wells over a one or more test plates that are instances of the template plate definition.
1 . A method for designing a cell-based screening assay experiment, the method comprising:
at a graphical user interface:
(1) receiving a first user input identifying a template plate definition for the cell-based screening assay experiment, the template plate definition including one or more pluralities of template test wells and at least one template control well;
(2) receiving a second user input defining (A) one or more sets of disease reagents associated with a first plurality of template test wells in the one or more pluralities of template test wells, and (B) at least one destination concentration for the one or more sets of disease reagents;
(3) receiving a third user input defining (A) a first set of treatment reagents, (B) at least one destination concentration for the first set of treatment reagents, (C) a number of replicates of the first set of treatment reagents, and (D) a selection to associate the first set of treatment reagents with a first set of disease reagents in the one or more sets of disease reagents;
automatically generating, based at least partially on the first user input, the second user input and the third user input, a design for the cell-based screening assay experiment, the automatically generating including:
(i) determining, from among the one or more pluralities of template test wells, a number of template test wells required for performing the cell-based screening assay experiment, thereby defining a first plurality of test wells in the design; and
(ii) distributing the first plurality of test wells over one or more test plates in the design, wherein each test plate in the one or more test plates is an instance of the template plate definition.
2 . The method of claim 1 , wherein:
the template plate definition includes a second plurality of template test wells in the one or more pluralities of template test wells;
the second user input further defines a second set of disease reagents in the one or more sets of disease reagents and a selection to associate the second set of disease reagents with the second plurality of template test wells in the one or more pluralities of template test wells.
3 . The method of claim 1 , wherein:
the third user input further defines (E) a second set of treatment reagents, (F) at least one destination concentration of the second set of treatment reagents, (G) a number of replicates of the second set of treatment reagents, and (H) a selection to associate the second set of treatment reagents with the first set of disease reagents.
4 . The method of claim 1 , further including;
receiving an additional user input adding one or more additional disease reagents to the first set of disease reagents, wherein:
the design for the cell-based screening assay experiment is automatically generated based on additionally, at least partially on, the additional user input.
5 . The method of claim 1 , wherein automatically generating the design for the cell-based screening assay experiment includes associating each test well of the first plurality of test wells with (1) a particular disease reagent of the first set of disease reagents in the one or more sets of disease reagents having a particular destination concentration of the at least one destination concentration of the first set of disease reagents, and (2) a particular treatment reagent of the first set of treatment reagents having a particular destination concentration of the at least one destination concentration for the first set of treatment reagents.
6 . The method of claim 1 , further comprising:
receiving an additional user input defining (A) a first set of control reagents associated with the at least one template control well and (B) at least one destination concentration of the first set of control reagents, wherein:
the design for the cell-based screening assay experiment is automatically generated based on additionally, at least partially on, the additional user input, the automatically generating further including:
(iii) determining a number of template control wells required for performing the cell-based screening assay experiment, thereby defining a first plurality of control wells; and
(iv) distributing the first plurality of control wells over the one or more test plates required for performing the cell-based screening assay experiment.
7 . The method of claim 6 , wherein automatically generating the design for the cell-based screening assay experiment includes associating each control well of the first plurality of control wells with a particular control reagent of the first set of control reagents having a particular concentration of the at least one destination concentration of the first set of control reagents.
8 . The method of claim 1 , wherein the second user input includes at least one source concentration and at least one respective transfer volume of the first set of disease reagents, and the at least one destination concentration of the first set of disease reagents is computed based on the at least one source concentration and the at least one respective transfer volume.
9 . A system for designing a cell-based screening assay experiment, the system comprising:
at least one processor and memory addressable by the at least one processor, the memory storing at least one program for execution by the at least one processor, the at least one program comprising instructions for:
displaying a graphical user interface;
at the graphical user interface:
(1) receiving a first user input identifying a template plate definition for the cell-based screening assay experiment, the template plate definition including one or more pluralities of template test wells and at least one template control well;
(2) receiving a second user input defining (A) one or more sets of disease reagents associated with a first plurality of template test wells in the one or more pluralities of template test wells, and (B) at least one destination concentration for the one or more sets of disease reagents;
(3) receiving a third user input defining (A) a first set of treatment reagents, (B) at least one destination concentration for the first set of treatment reagents, (C) a number of replicates of the first set of treatment reagents, and (D) a selection to associate the first set of treatment reagents with a first set of disease reagents in the one or more sets of disease reagents;
automatically generating, based at least partially on the first user input, the second user input and the third user input, a design for the cell-based screening assay experiment, the automatically generating including:
(i) determining, from among the one or more pluralities of template test wells, a number of template test wells required for performing the cell-based screening assay experiment, thereby defining a first plurality of test wells in the design; and
(ii) distributing the first plurality of test wells over one or more test plates in the design, wherein each test plate in the one or more test plates is an instance of the template plate definition.
10 . The system of claim 9 , wherein:
the template plate definition includes a second plurality of template test wells in the one or more pluralities of template test wells;
the second user input further defines a second set of disease reagents in the one or more sets of disease reagents and a selection to associate the second set of disease reagents with the second plurality of template test wells in the one or more pluralities of template test wells.
11 . The system of claim 9 , wherein:
the third user input further defines (E) a second set of treatment reagents, (F) at least one destination concentration of the second set of treatment reagents, (G) a number of replicates of the second set of treatment reagents, and (H) a selection to associate the second set of treatment reagents with the first set of disease reagents.
12 . The system of claim 9 , wherein the at least one program further includes instructions for:
receiving an additional user input adding one or more additional disease reagents to the first set of disease reagents, and wherein:
the design for the cell-based screening assay experiment is automatically generated based on additionally, at least partially on, the additional user input.
13 . The system of claim 9 , wherein:
wherein automatically generating the design for the cell-based screening assay experiment includes associating each test well of the first plurality of test wells with (1) a particular disease reagent of the first set of disease reagents in the one or more sets of disease reagents having a particular destination concentration of the at least one destination concentration of the first set of disease reagents, and (2) a particular treatment reagent of the first set of treatment reagents having a particular destination concentration of the at least one destination concentration for the first set of treatment reagents.
14 . The system of claim 9 , wherein the at least one program further includes instructions for:
receiving an additional user input defining (A) a first set of control reagents associated with the at least one template control well and (B) at least one destination concentration of the first set of control reagents, wherein:
the design for the cell-based screening assay experiment is automatically generated based on additionally, at least partially on, the additional user input, the automatically generating further including:
(iii) determining a number of template control wells required for performing the cell-based screening assay experiment, thereby defining a first plurality of control wells; and
(iv) distributing the first plurality of control wells over the one or more test plates required for performing the cell-based screening assay experiment.
15 . The system of claim 14 , wherein automatically generating the design for the cell-based screening assay experiment includes associating each control well of the first plurality of control wells with a particular control reagent of the first set of control reagents having a particular concentration of the at least one destination concentration of the first set of control reagents.
16 . The system of claim 9 , wherein the second user input includes at least one source concentration and at least one respective transfer volume of the first set of disease reagents, and the at least one destination concentration of the first set of disease reagents is computed based on the at least one source concentration and the at least one respective transfer volume.
17 . A non-transitory computer readable media storing one or more programs, the one or more programs comprising instructions, which when executed by an electronic device with a display cause the electronic device to:
display a graphical user interface;
at the graphical user interface:
(1) receive a first user input identifying a template plate definition for the cell-based screening assay experiment, the template plate definition including one or more pluralities of template test wells and at least one template control well;
(2) receive a second user input defining (A) one or more sets of disease reagents associated with a first plurality of template test wells in the one or more pluralities of template test wells, and (B) at least one destination concentration for the one or more sets of disease reagents;
(3) receive a third user input defining (A) a first set of treatment reagents, (B) at least one destination concentration for the first set of treatment reagents, (C) a number of replicates of the first set of treatment reagents, and (D) a selection to associate the first set of treatment reagents with a first set of disease reagents in the one or more sets of disease reagents;
automatically generate, based at least partially on the first user input, the second user input and the third user input, a design for the cell-based screening assay experiment, the automatically generating including:
(i) determining, from among the one or more pluralities of template test wells, a number of template test wells required for performing the cell-based screening assay experiment, thereby defining a first plurality of test wells in the design; and
(ii) distributing the first plurality of test wells over one or more test plates in the design, wherein each test plate in the one or more test plates is an instance of the template plate definition.
18 . The non-transitory computer readable media of claim 17 , wherein:
the template plate definition includes a second plurality of template test wells in the one or more pluralities of template test wells;
the second user input further defines a second set of disease reagents in the one or more sets of disease reagents and a selection to associate the second set of disease reagents with the second plurality of template test wells in the one or more pluralities of template test wells.
19 . The non-transitory computer readable media of claim 17 , wherein:
the third user input further defines (E) a second set of treatment reagents, (F) at least one destination concentration of the second set of treatment reagents, (G) a number of replicates of the second set of treatment reagents, and (H) a selection to associate the second set of treatment reagents with the first set of disease reagents.
20 . The non-transitory computer readable media of claim 17 , wherein the one or more programs further include instructions for:
receiving an additional user input adding one or more additional disease reagents to the first set of disease reagents, and wherein:
the design for the cell-based screening assay experiment is automatically generated based on additionally, at least partially on, the additional user input.