Analyte sensors featuring working electrode asperity planing for decreasing interferent signal
Analyte sensors are being increasingly employed for monitoring various analytes in vivo. Analyte sensors may feature enhancements to address signals obtained from interferent species. Some analyte sensors may comprise an analyte sensor comprising a working electrode comprising an active area disposed thereon and electrode asperities laser planed therefrom, the active area comprising an analyte-responsive enzyme. Methods include laser singulating a working electrode, the working electrode comprising an active area disposed thereupon and electrode asperities, the active area comprising an analyte-responsive enzyme, and laser planing at least a portion of the electrode asperities.
1 . A method of creating a laser planed working electrode comprising:
laser singulating a working electrode from a template carbon substrate material into a shape, the working electrode comprising an active area disposed thereon, the active area having an analyte-responsive enzyme,
wherein the laser singulated working electrode comprises electrode asperities at an outermost edge thereof; and
laser planing at least a portion of the outermost edge to remove at least a portion of the electrode asperities at the outermost edge,
wherein the laser planing comprises making a plurality of spaced apart cuts between a midline length and the outermost edge of the laser singulated working electrode to create an outer beveled edge of the shape, thereby resulting in the laser planed working electrode, wherein a depth of each of the plurality of spaced apart cuts is less than a thickness of the working electrode.
2 . The method of claim 1 , wherein the laser planed working electrode exhibits a reduction in interferent signal of an interferent compared to a working electrode that has not been planed.
3 . The method of claim 2 , wherein the reduction in interferent signal of the interferent is greater than 20%.
4 . The method of claim 2 , wherein the interferent is ascorbic acid.
5 . The method of claim 1 , further comprising removing at least 5% of a total area of the electrode asperities.
6 . The method of claim 1 , wherein the laser planing comprises a plurality of single-pass laser planing cuts.
7 . The method of claim 6 , wherein the plurality of single-pass laser planing cuts are at least one of perpendicular to the outermost edge of the laser singulated working electrode or beveled relative to the outermost edge of the laser singulated working electrode.
8 . The method of claim 1 , wherein the electrode asperities have a width in the range of 1 μm to 75 μm and a height of 1 μm to 50 μm.
9 . The method of claim 1 , wherein the active area is comprised of a plurality of discontiguous active areas.
10 . The method of claim 1 , wherein the active area is comprised of a single contiguous active area.
11 . The method of claim 1 , wherein the active area is compressed.
12 . The method of claim 1 , wherein the analyte-responsive enzyme is a glucose-responsive enzyme.
13 . The method of claim 1 , wherein a membrane is disposed upon at least a portion of the active area.
14 . The method of claim 1 , wherein the plurality of spaced apart cuts comprises at least cuts at 20 μm, 40 μm, and 60 μm from the outermost edge of the laser singulated working electrode.
15 . An analyte sensor comprising:
a working electrode comprising an active area disposed thereon, the active area having an analyte-responsive enzyme,
wherein the working electrode is first laser singulated from a template carbon substrate material, thereby resulting in electrode asperities at an edge thereof, and
thereafter the edge of the working electrode is laser planed to remove at least a portion of the electrode asperities therefrom at the edge, thereby resulting in a laser planed working electrode,
wherein the laser singulated working electrode is laser planed by making a plurality of spaced apart cuts between a midline length and an outermost edge of the working electrode to create an outer beveled edge, wherein a depth of each of the plurality of spaced apart cuts is less than a thickness of the working electrode.
16 . The analyte sensor of claim 15 , wherein the laser planed working electrode exhibits a reduction in interferent signal of an interferent compared to a working electrode that has not been planed.
17 . The analyte sensor of claim 16 , wherein the reduction in interferent signal of the interferent is greater than 20%.
18 . The analyte sensor of claim 16 , wherein the interferent is ascorbic acid.
19 . The analyte sensor of claim 15 , wherein at least 5% of a total area of the electrode asperities is removed.
20 . The analyte sensor of claim 15 , wherein the electrode asperities have a width in the range of 1 μm to 75 μm and have a height of 1 μm to 50 μm.
21 . The analyte sensor of claim 15 , wherein the active area is comprised of a plurality of discontiguous active areas.
22 . The analyte sensor of claim 15 , wherein the active area is comprised of a single contiguous active area.
23 . The analyte sensor of claim 15 , wherein the active area is compressed.
24 . The analyte sensor of claim 15 , wherein the laser planing comprises at least cuts at 20 μm, 40 μm, and 60 μm from the outermost edge of the working electrode.