Pulse control method and apparatus, ablation device and system, and storage medium

1 . A pulse control method, comprising:

controlling a pulse generator to output a nanosecond pulse sequence, and

controlling the pulse generator to output a millisecond pulse sequence,

wherein an amplitude of the nanosecond pulse sequence is greater than a preset first threshold voltage, and an amplitude of the millisecond pulse sequence is less than a preset second threshold voltage, and

wherein the nanosecond pulse sequence causes irreversible electroporation to occur in cells close to an electrode needle such that the cells enter an apoptosis process, and causes reversible electroporation to occur in cells relatively far from the electrode needle;

wherein the millisecond pulse sequence causes intracellular electrolysis of the cells relatively far from the electrode needle in which reversible electroporation occurs, such that the cells relatively far from the electrode needle also enter the apoptosis process.

2 . The pulse control method according to claim 1 , wherein controlling the pulse generator to output the nanosecond pulse sequence and controlling the pulse generator to output the millisecond pulse sequence comprises:

alternatively outputting the nanosecond pulse sequence and the millisecond pulse sequence.

3 . The pulse control method according to claim 2 , wherein alternatively outputting the nanosecond pulse sequence and the millisecond pulse sequence comprises:

each time it is determined that one nanosecond pulse in the nanosecond pulse sequence has been outputted, outputting one millisecond pulse in the millisecond pulse sequence; and/or

each time it is determined that one millisecond pulse in the millisecond pulse sequence has been outputted, outputting one nanosecond pulse in the nanosecond pulse sequence.

4 . The pulse control method according to claim 2 , wherein alternatively outputting the nanosecond pulse sequence and the millisecond pulse sequence comprises:

each time it is determined that at least some nanosecond pulses in the nanosecond pulse sequence have been outputted, outputting at least some millisecond pulses in the millisecond pulse sequence; and/or

each time it is determined that at least some millisecond pulses in the millisecond pulse sequence have been outputted, outputting at least some nanosecond pulses in the nanosecond pulse sequence.

5 . The pulse control method according to claim 2 , wherein alternatively outputting the nanosecond pulse sequence and the millisecond pulse sequence comprises:

each time it is determined that at least some nanosecond pulses in the nanosecond pulse sequence have been outputted, outputting one millisecond pulse in the millisecond pulse sequence;

and/or each time it is determined that at least some millisecond pulses in the millisecond pulse sequence have been outputted, outputting one nanosecond pulse in the nanosecond pulse sequence.

6 . The pulse control method according to claim 3 , wherein a set interval exists between at least one nanosecond pulse in the nanosecond pulse sequence and an adjacent millisecond pulse in the millisecond pulse sequence.

7 . The pulse control method according to claim 6 , wherein the set interval is not shorter than 1 nanosecond and not longer than 1 second.

8 . The pulse control method according to claim 3 , wherein a falling edge of at least one nanosecond pulse in the nanosecond pulse sequence corresponds to a rising edge of an adjacent millisecond pulse in the millisecond pulse sequence; and/or

a rising edge of at least one nanosecond pulse in the nanosecond pulse sequence corresponds to a falling edge of an adjacent millisecond pulse in the millisecond pulse sequence.

9 . The pulse control method according to claim 1 , wherein a set interval exists between an ending time of at least one nanosecond pulse in the nanosecond pulse sequence and a starting time of a following adjacent millisecond pulse in the millisecond pulse sequence, and the set interval is not longer than 1 second.

10 . The pulse control method according to claim 1 , wherein the nanosecond pulse sequence includes one or more of the following features:

a frequency of the nanosecond pulse sequence being not less than 0.1 Hz and not greater than 10 Hz;

the nanosecond pulse sequence comprising no less than 2 nanosecond pulses and no more than 5000 nanosecond pulses;

a pulse width of at least one nanosecond pulse in the nanosecond pulse sequence being not less than 10 nanoseconds and not greater than 1000 nanoseconds;

an amplitude of at least one nanosecond pulse in the nanosecond pulse sequence being not less than 5 kV and not greater than 100 kV;

the nanosecond pulse sequence being a square wave pulse sequence; and

the nanosecond pulse sequence being a bipolar pulse sequence.

11 . The pulse control method according to claim 1 , wherein the millisecond pulse sequence includes one or more of the following features:

a frequency of the millisecond pulse sequence being not less than 0.1 Hz and not greater than 10 Hz;

the millisecond pulse sequence comprising no less than 2 millisecond pulses and no more than 5000 millisecond pulses;

a pulse width of at least one millisecond pulse in the millisecond pulse sequence being not less than 1 ms and not greater than 1000 ms;

an amplitude of at least one millisecond pulse in the millisecond pulse sequence being not less than 5 V and not greater than 100 V;

the millisecond pulse sequence being a square wave pulse sequence; and

the millisecond pulse sequence being a bipolar pulse sequence.

12 . A pulse control apparatus, comprising:

an electric pulse control module configured to control a pulse generator to output a nanosecond pulse sequence and control the pulse generator to output a millisecond pulse sequence; and

control an amplitude of the nanosecond pulse sequence to be greater than a preset first threshold voltage and control an amplitude of the millisecond pulse sequence to be less than a preset second threshold voltage, and

wherein the nanosecond pulse sequence causes irreversible electroporation to occur in cells close to an electrode needle such that the cells enter an apoptosis process, and causes reversible electroporation to occur in cells relatively far from the electrode needle;

wherein the millisecond pulse sequence causes intracellular electrolysis of the cells relatively far from the electrode needle in which reversible electroporation occurs, such that the cells relatively far from the electrode needle also enter the apoptosis process.

13 . An ablation device, comprising:

an electrode needle configured to contact a target object and output an electric pulse to the target object, the electric pulse comprising a nanosecond pulse sequence and a millisecond pulse sequence;

a pulse generator electrically connected to the electrode needle and configured to generate the electric pulse and conduct the electric pulse to the electrode needle; and

a controller communicatively connected with the pulse generator and configured to perform a pulse control method comprising:

controlling the pulse generator to output the nanosecond pulse sequence, and

controlling the pulse generator to output the millisecond pulse sequence,

wherein an amplitude of the nanosecond pulse sequence is greater than a preset first threshold voltage, and an amplitude of the millisecond pulse sequence is less than a preset second threshold voltage, and

wherein the nanosecond pulse sequence causes irreversible electroporation to occur in cells close to the electrode needle such that the cells enter an apoptosis process, and causes reversible electroporation to occur in cells relatively far from the electrode needle;

wherein the millisecond pulse sequence causes intracellular electrolysis of the cells relatively far from the electrode needle in which reversible electroporation occurs, such that the cells relatively far from the electrode needle also enter the apoptosis process.

14 . The ablation device according to claim 13 , wherein the pulse generator comprises:

a first sub-generator electrically connected to the electrode needle, communicatively connected with the controller and configured to generate the nanosecond pulse sequence; and

a second sub-generator electrically connected to the electrode needle, communicatively connected with the controller and configured to generate the millisecond pulse sequence.

15 . An ablation system comprising the ablation device according to claim 13 and an upper computer,

the upper computer being communicatively connected with the controller in the ablation device.

16 . A non-transitory computer-readable storage medium having a computer program stored therein, wherein the computer program, when executed by a processor, implements the pulse control method according to claim 1 .

17 . The method according to claim 1 , wherein,

controlling the pulse generator to output the nanosecond pulse sequence, and controlling the pulse generator to output the millisecond pulse sequence, comprises:

controlling the pulse generator to first output the nanosecond pulse sequence to induce occurrence of reversible cell electropores in the cells relatively far from the electrode needle, and then during existence of at least some of the reversible cell electropores, to output the millisecond pulse sequence.