Substance delivery carrier and composition
According to one embodiment, a substance delivery carrier and a composition are used for delivering an intended substance to a myeloid tumor cell. The substance delivery carrier has a lipid particle, and an intended substance encapsulated in the lipid particle. The lipid particle contains, as a constituent thereof, at least a first lipid represented by formula (I).
1 . A method for delivering a substance to a myeloid tumor cell, the method comprising:
preparing a substance delivery carrier which comprises a lipid particle and the substance encapsulated in the lipid particle; and
contacting the substance delivery carrier with the myeloid tumor cell;
wherein the lipid particle contains a first lipid represented by formula (I):
and at least one of cationic lipid, neutral lipid, anti-aggregation lipid, and cholesterol,
wherein
a mole ratio of the first lipid in a lipid composition of the lipid particle is from 10% to 50%, and
the lipid particle exhibits 8.4 pKa or smaller, in an environment with an ionic strength of 3.599×10 −3 mol/dm 3 .
2 . The method of claim 1 , wherein the substance contains siRNA that suppresses expression of a gene relevant to onset and progression of the myeloid tumor cell.
3 . The method of claim 2 , wherein the siRNA contains a sequence complementary to a part of the gene.
4 . The method of claim 2 , wherein the gene is at least one selected from the group consisting of transcription factor, fusion gene, epigenome control factor, phosphorylating enzyme-related gene, immunosuppressive factor, a ubiquitin-related factor, apoptosis control factor, and non-coding RNA.
5 . The method of claim 2 , wherein the gene is at least one selected from RUNX1, RUNX2, MECOM, MYB, MYC, HOXA9, MEIS1, HHEX, and ERG.
6 . The method of claim 1 , wherein the lipid particle further encapsulates at least either a pH adjustor or an osmoregulator.
7 . The method of claim 1 , wherein
the myeloid tumor cell exists in vivo in a subject, and
contacting is carried out by administering a composition that contains the substance delivery carrier to the subject.
8 . A substance delivery carrier for delivering a substance to a myeloid tumor cell, the substance delivery carrier comprising:
a lipid particle; and
the substance encapsulated in the lipid particle,
the lipid particle containing at least a first lipid represented by formula (I):
and at least one of cationic lipid, neutral lipid, anti-aggregation lipid, and cholesterol
wherein
a mole ratio of the first lipid in a lipid composition of the lipid particle is from 10% to 50%, and
the lipid particle exhibits 8.4 pKa or smaller, in an environment with an ionic strength of 3.599×10−3 mol/dm3.
9 . The substance delivery carrier of claim 8 , wherein the lipid particle is weakly cationic.
10 . The substance delivery carrier of claim 8 , wherein the substance contains siRNA that suppresses expression of a gene relevant to onset and progression of the myeloid tumor cell.
11 . The substance delivery carrier of claim 10 , wherein the siRNA contains a sequence complementary to a part of the gene.
12 . The substance delivery carrier of claim 10 , wherein the gene is at least one selected from the group consisting of transcription factor, fusion gene, epigenome control factor, phosphorylating enzyme-related gene, immunosuppressive factor, a ubiquitin-related factor, apoptosis control factor, and non-coding RNA.
13 . The substance delivery carrier of claim 10 , wherein
the gene is at least one selected from RUNX1, RUNX2, MECOM, MYB, MYC, HOXA9, MEIS1, HHEX, and ERG.
14 . The substance delivery carrier of claim 8 , wherein the lipid particle further encapsulates at least either pH adjustor or an osmoregulator.
15 . A composition comprising the substance delivery carrier of claim 8 ,
and further comprising at least either a carrier or a storage protectant.
16 . The composition of claim 15 , wherein the storage protectant is present and is dimethylsulfoxide.