Processes and apparatuses for obtaining amniotic mesenchymal stem cells from amniotic fluid and cells derived thereof
Methods for purifying, culturing and selecting mesenchymal stem cell (MSC) subpopulations with neonatal quality and adult tissue specificity that are for use in production of advanced therapeutic medicinal products.
1 . A method for obtaining term amniotic fluid lung mesenchymal stem cells (TAF lung MSCs) from term amniotic fluid, comprising:
providing term amniotic fluid (TAF);
removing particulate material from the TAF to obtain purified TAF cells;
performing adherence selection on the purified TAF cells to obtain TAF adherence cells;
passaging the TAF adherence cells to obtain a population of cells comprising the lung TAF MSCs; and
selecting the TAF lung MSCs from the population as cells that express Group B surface markers DDR1, CD248 and LRRC38, thereby obtaining TAF lung mesenchymal stem cells.
2 . The method of claim 1 , wherein selecting lung TAF MSCs further comprises excluding MSCs that express a marker selected from the group consisting of CD24, ITGB4, TNFSF10, GFRA1, CD74, FGFR4, HAVCR1, and OSCAR.
3 . The method of claim 1 , wherein the selecting step further comprises selecting TAF MSCs that further express at least another B surface marker selected from the group consisting of PCDH19, MME, IFITM10, BGN, NOTCH3, SULF1, TNFSF18, BDKRB1, FLT1, PDGFRA, TNFSF4, UNC5B, FAP, CASP1, DDR2, PCDH18 and CRLF1.