Methods of reducing collagenase-mediated bruising in a subject having cellulite
Disclosed herein are methods of reducing collagenase-mediated bruising in a subject having cellulite, methods of treating cellulite in a subject, and collagenase-containing formulations.
1 . A method of reducing bruising and/or skin discoloration associated with collagenase-mediated treatment of cellulite in a subject, the method comprising:
subcutaneously administering to a treatment area of the subject a total dose of about 0.42 mg of collagenase per treatment session to thereby reduce the bruising and/or skin discoloration associated with the collagenase-mediated treatment of cellulite in the subject, wherein the subject experiences an at least 1 level improvement on an Investigator Assessment of Bruising Severity Scale (IABSS).
2 . The method of claim 1 , wherein the collagenase is administered using a three-injection technique.
3 . The method of claim 2 , wherein the collagenase is administered at a depth of about 0.5 inches.
4 . The method of claim 1 , wherein the collagenase has a concentration of about 0.23 mg/ml.
5 . The method of claim 1 , wherein the subject receives multiple treatment sessions.
6 . The method of claim 5 , wherein the treatment sessions are 3 weeks apart.
7 . The method of claim 1 , wherein the collagenase is administered in a composition comprising the collagenase, mannitol, sucrose, and tromethamine.
8 . The method of claim 1 , wherein the collagenase comprises a collagenase I, a collagenase II, or a mixture of collagenase I and collagenase II.
9 . The method of claim 8 , wherein the collagenase comprises the mixture of collagenase I and collagenase II.
10 . The method of claim 9 , wherein the collagenase I comprises the amino acid sequence of SEQ ID NO: 1 and the collagenase II comprises the amino acid sequence of SEQ ID NO: 2.
11 . The method of claim 8 , wherein the collagenase is collagenase Clostridium histolyticum (CCH).