IP Library › Granted Patent US 12667825
Granted Patent B2
US 12667825 · App. 18/112,806 · Granted Jun 30, 2026

Chromatographic column

Inventors: Ahmed Yacine Badjah Hadj Ahmed (Riyadh, SA); Mohamed Ali Ouladsmane (Riyadh, SA); Taieb Aouak (Riyadh, SA); Zeid Abdullah Alothman (Riyadh, SA)
Assignee: KING SAUD UNIVERSITY
B01J20/286B01D15/22B01J20/267B01J20/28042B01J20/3092B01J20/3219B01J2220/58B01J2220/82B01J2220/84B01J2220/86
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Quick Facts
Patent No.
US 12667825
App. No.
18/112,806
Granted
Jun 30, 2026
Kind
B2
Abstract

The chromatographic column is a stainless steel column having a stationary phase of porous monolithic polymer chemically bonded to the interior wall of the stainless steel column so that the stationary phase is fixed and immobile and does not require frits or other seals to retain particulate matter or a slurry in the column. The inner wall of the column is oxidized and then vinylized with a difunctional linker to chemically bond the polymer to the column. The difunctional linker may be 3-(trimethoxysilyl) propyl methacrylate, vinyl trimethoxysilane, 3-mercaptopropyl trimethoxysilane, or glycidoxypropyltrimethoxysilane. The monolithic polymer is prepared in situ in the column by radical polymerization of a monovinyl monomer with a crosslinking divinyl monomer. The column may be a conventional stainless steel column (¼″ inner diameter), capillary micro-LC ( 1/16″), or nan-LC (( 1/32″).

Claims (13)

1 . A chromatographic column, comprising:

a tubular stainless steel column having an inner surface; and

a porous monolithic polymer stationary phase chemically bonded to the inner surface of the stainless steel column;

whereby the stationary phase is fixed within the stainless steel column without frits and other seals to retain the stationary phase within the stainless steel column.

2 . The chromatographic column according to claim 1 , further comprising a difunctional linking compound bonded to the inner surface of said stainless steel column, said porous monolithic polymer being bonded to the difunctional linker compound.

3 . The chromatographic column according to claim 2 , wherein said difunctional linking compound is selected from the group consisting of 3-(trimethoxysilyl) propyl methacrylate, vinyl trimethoxysilane, 3-mercaptopropyl trimethoxysilane, and glycidoxypropyltrimethoxysilane.

4 . The chromatographic column according to claim 1 , wherein said porous monolithic polymer comprises a monovinyl monomer crosslinked with a divinyl monomer.

5 . The chromatographic column according to claim 4 , wherein said monovinyl monomer is selected from the group consisting of an acrylate, a methacrylate, a styrene, an acrylamide, and a methacrylamide.

6 . The chromatographic column according to claim 4 , wherein said divinyl monomer is selected from the group consisting of divinylbenzene, ethylene dimethacrylate, glycerol dimethacrylate, N,N-methylene bisacrylamide, poly (ethylene) diacrylate, and bisphenol A dimethacrylate.

7 . The chromatographic column according to claim 1 , wherein said porous monolithic polymer comprises polystyrene crosslinked with divinyl benzene.

8 . The chromatographic column according to claim 1 , wherein said stainless steel column has an outer diameter of ¼″.

9 . The chromatographic column according to claim 1 , wherein said stainless steel column is a micro-capillary liquid chromatography column having an outer diameter of 1/16″.

10 . The chromatographic column according to claim 1 , wherein said stainless steel column is a nano-capillary liquid chromatography column having an outer diameter of 1/32″.