Micro-fluidic chip, library preparation chip and method for controlling and driving droplet
A micro-fluidic chip is provided. The micro-fluidic chip includes: a first base substrate; a first electrode on the first base substrate and electrically coupled to a wire at a driving end; a second electrode on a side of the first electrode away from the first base substrate and spaced apart and electrically insulated from the first electrode, the second electrode including a plurality of sub-blocks of the second electrode, and an orthographic projection of the second electrode on the first base substrate being at least partially overlapped with an orthographic projection of the first electrode on the first base substrate; and voltage-dividing resistors coupled to the plurality of sub-blocks of the second electrode in one-to-one correspondence and electrically coupled to a ground wire.
1 . A micro-fluidic chip, comprising:
a first base substrate;
a first electrode on the first base substrate and electrically coupled to a wire at a driving end;
a second electrode on a side of the first electrode away from the first base substrate and spaced apart and electrically insulated from the first electrode, the second electrode comprising a plurality of sub-blocks of the second electrode, and an orthographic projection of the second electrode on the first base substrate being at least partially overlapped with an orthographic projection of the first electrode on the first base substrate; and
voltage-dividing resistors electrically coupled in parallel with each other, wherein each of the voltage-dividing resistors is coupled between a corresponding one of the plurality of sub-blocks of the second electrode and a ground wire,
wherein the micro-fluidic chip further comprises a dielectric layer on a side of the second electrode away from the first base substrate and a second lyophobic layer on a side of the dielectric layer away from the first base substrate and the second lyophobic layer is configured to support a droplet and is in direct contact with the droplet,
wherein resistance values of the voltage-dividing resistors gradually increase in a first direction, and voltages between the plurality of sub-blocks of the second electrode and the ground wire gradually increase in the first direction in proportion to the resistance values of the voltage-dividing resistors, and
wherein each of the voltage-dividing resistors is formed by a conductive line having a square-wave shape, and lengths of the conductive lines forming the voltage-dividing resistors are different from one another and gradually increase in the first direction.
2 . The micro-fluidic chip of claim 1 , wherein
an insulating layer is disposed between the first electrode and the second electrode, and a thickness of the insulating layer is uniform in a region corresponding to the first electrode; and
overlapping areas of the orthographic projection of the first electrode on the first base substrate and the orthographic projection of the second electrode on the first base substrate are the same.
3 . The micro-fluidic chip of claim 2 , wherein
the first electrode comprises a plurality of sub-blocks of the first electrode spaced apart from each other in the first direction, having the same shape and coupled to each other, and each of the plurality of sub-blocks of the first electrode has a same shape as each of the plurality of sub-blocks of the second electrode; and
an orthographic projection of a sub-block of the plurality of sub-blocks of the first electrode on the first base substrate and an orthographic projection of a respective sub-block of the plurality of sub-blocks of the second electrode on the first base substrate coincide.
4 . The micro-fluidic chip of claim 3 , wherein
the plurality of sub-blocks of the second electrode are equally spaced along the first direction.
5 . The micro-fluidic chip of claim 1 , further comprising a third electrode coupled to the first electrode.
6 . The micro-fluidic chip of claim 5 , wherein the third electrode and the second electrode are disposed in a same layer, an insulating layer is disposed between the first electrode and a fourth electrode, and the third electrode is coupled to the first electrode through a first via hole penetrating through the insulating layer.
7 . The micro-fluidic chip of claim 1 , wherein the ground wire and the first electrode are disposed in a same layer, the voltage-dividing resistors and the second electrode are disposed in a same layer, an insulating layer is disposed between the voltage-dividing resistors and the ground wire, and the ground wire is coupled to the voltage-dividing resistors one by one through a second via hole penetrating through the insulating layer.
8 . The micro-fluidic chip of claim 1 , wherein a number of the plurality of sub-blocks of the second electrode is from 2 to 4.
9 . The micro-fluidic chip of claim 1 , wherein
the micro-fluidic chip further comprises a third electrode coupled to the first electrode, the third electrode and the second electrode are disposed in a same layer, an insulating layer is disposed between the first electrode and the third electrode and the second electrode disposed in the same layer, and the third electrode is coupled to the first electrode through a first via hole penetrating through the insulating layer, and
the third electrode is configured to receive a signal such that a droplet above the second electrode is driven through coupling effect between the first electrode and the second electrode due to the signal.
10 . The micro-fluidic chip of claim 1 , wherein each of the voltage-dividing resistors is directly coupled between the corresponding one of the plurality of sub-blocks of the second electrode and the ground wire.
11 . A library preparation chip, comprising the micro-fluidic chip of claim 1 , and further comprising a sample distribution module, a reagent distribution module, a mixing module, a reaction module, a washing module, and a sample output module, wherein the sample distribution module, the reagent distribution module and the sample output module are coupled to other modules through a structure of the micro-fluidic chip; and each of the mixing module, the reaction module and the washing module comprises a structure of the micro-fluidic chip.
12 . A method for controlling and driving a droplet to quickly transport the droplet, applied to the micro-fluidic chip of claim 1 , comprising:
providing a signal to the first electrode to drive the droplet to move.
13 . The method of claim 12 , wherein the signal is an alternating current signal having an effective value from 10 Vrms to 70 Vrms.