Cell screening device and cell screening kit
A cell screening device having a bottom plate part; a cell placement membrane provided on the bottom plate part and forming a cell placement surface; a pair of fluid injection parts provided on the bottom plate part and partitioned from the cell placement membrane; and a flow channel provided between the bottom plate part and the cell placement membrane and having flow channel end portions extending to the fluid injection parts. On the cell placement surface of the cell placement membrane are formed a plurality of wells having a size that enables the wells to individually accommodate cells to be screened, and through holes extending from the inner bottom surfaces of the wells and communicating with the flow channel.
1 . A cell screening device, comprising:
a bottom plate having a rectangular shape and having a spacer formed in an upper part thereof;
an outer frame body that has a peripheral wall, the peripheral wall comprising a rectangular wall portion in plan view and a pair of end wall portions, the outer frame body being detachably attached to an upper surface of the bottom plate;
a tubular frame body having a rectangular shape in plan view that is detachably accommodated in the rectangular wall portion of the peripheral wall of the outer frame body;
a cell placement membrane that is supported by the outer frame body on the spacer of the bottom plate so as to form a flow channel between the cell placement membrane and the bottom plate and has a cell placement surface thereon;
a pair of lid portions, each lid portion being provided in the outer frame body apart from the tubular frame body and the cell placement membrane, each lid portion being formed on a side of the cell placement membrane on the bottom plate in a longitudinal direction of the bottom plate;
a pair of flow channel end parts formed between each of the lid portions and the bottom plate and communicating with the flow channel;
a pair of fluid injection ports, each fluid injection port being provided to stand on an upper surface of one of the lid portions and having an opening communicating with its respective flow channel end part through its respective lid portion; and
a plurality of wells formed in the cell placement membrane to open on the cell placement surface and having a size in which cells to be screened are individually accommodatable;
a through-hole that is formed in an inner bottom surface of each of the wells to reach the flow channel and has an inner diameter through which the cells to be screened are not passable;
a fluid storage that is formed lower than the opening of the fluid injection port on the upper surface of the lid around the fluid injection port so as to store a fluid that overflows from the opening of the fluid injection port; and
a partition that is formed in the outer frame body between the tubular frame body and the lid so as to prevent the fluid stored in the fluid storage from flowing to the cell placement membrane,
wherein the tubular frame body has an engagement groove that is formed on an outer circumference lower end surface of the tubular frame body so as to open upward, the outer frame body has an engagement ridge that is formed on a lower end of the outer frame body so as to protrude downward, and the engagement ridge is inserted into the engagement groove so that the tubular frame body is detachable downward with respect to the outer frame body.
2 . The cell screening device according to claim 1 ,
wherein the plurality of the wells are formed in the cell placement membrane in a lattice form,
the cell placement membrane is stretched over the lower end of the tubular frame body, and
an engaging part is provided on the outer frame body for detachably fixing the first tubular frame body on the bottom plate.
3 . The cell screening device according to claim 2 ,
wherein the outer frame body has a space for accommodating the tubular frame body, and
the tubular frame body that supports the membrane body is detachably fixed inside the space of the outer frame body.
4 . The cell screening device according to claim 1 ,
wherein the fluid injection port has a cylindrical shape.
5 . The cell screening device according to claim 1 ,
wherein each lid portion has a bubble discharge surface formed on at least a part of a lower surface of the lid that is a ceiling surface of the flow channel end, the bubble discharge surface rises in an inclined surface shape or a stepped shape approaching the fluid injection port.