IP Library Granted Patent US 12668614
Granted Patent B2
US 12668614 · App. 18/629,890 · Granted Jun 30, 2026

Use of

Inventors: Deyue Yu (Nanjing, CN); Guizhen Kan (Nanjing, CN); Wenjie Yuan (Nanjing, CN)
Assignee: Nanjing Agricultural University
C07K14/415C12N15/8242C12N15/8261
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Quick Facts
Patent No.
US 12668614
App. No.
18/629,890
Granted
Jun 30, 2026
Kind
B2
Abstract

The present disclosure relates to the technical field of genetic engineering, and in particular to a use of a Glycine max GmSAMMT gene in regulation of a protein content and/or a yield of a plant. The present disclosure has proved through experiments that an expression level of the GmSAMMT gene in a GmSAMMT-knockout line is significantly lower than an expression level of the GmSAMMT gene in a control line, and a protein content and a seed weight of a GmSAMMT-knockout line are significantly higher than a protein content and a seed weight of a control line. Compared with the control line, in a GmSAMMT-overexpressed line, a transcription level of the GmSAMMT gene is significantly increased, a protein content is significantly reduced, and a seed weight is also reduced without a significant difference.

Claims (4)

1 . A method for breeding a transgenic Glycine max plant, comprising

constructing a recombinant vector for knockout of a GmSAMMT gene; and

transforming the recombinant vector into a Glycine max plant to obtain the transgenic Glycine max plant;

wherein the protein encoded by the GmSAMMT gene has the amino acid sequence of SEQ ID NO: 1 and the transgenic Glycine max plant has a higher seed protein content and higher yield than those of a control plant.