Microfluidic system simulating lung tissue
Disclosed are a biomimic system simulating lung tissue, a method for manufacturing same, and a microfluidic control method using same, wherein the biomimic system comprises lung epithelial cells and lung fibroblasts, which are isolated from human lungs, and commercially available vascular endothelial cells, and wherein a microfluid flows through the biomimic system. Each chamber inside the corresponding system can allow a fluid, which contains gas and a medium, to flow therethrough and simulate respiration-like movement, wherein all of the three types of cells can survive inside the system even when one week or more have elapsed after through-flow of the fluid. In addition, the pH and pO 2 in the chamber can be monitored by using a pH sensor and a gas partial pressure sensor inside the system, and thus the three types of cells inside the system can be exposed to external environments, drugs, and the like under the same conditions as in the lungs in vivo. Therefore, a wide range of studies including modeling of lung diseases by harmful substances and testing of therapeutic drug efficacy can be conducted, and further, the utilization to in vitro disease modeling, customized medicine prescriptions, and the like can also be made.
1 . A microfluidic control method in a microfluidic system simulating a lung tissue, the system comprising:
a first layer; a second layer; a third layer;
a first chamber for gas perfusion between the first layer and the second layer; and
a second chamber for medium-containing fluid perfusion between the second layer and the third layer,
wherein the second layer includes a porous membrane,
the porous membrane includes lung epithelial cells, lung fibroblasts, and vascular endothelial cells,
the lung epithelial cells face the first chamber,
the vascular endothelial cells face the second chamber; the method comprising
a microfluidic perfusion step of perfusing gas to the first chamber of the microfluidic system and perfusing the medium-containing fluid to the second chamber,
wherein the porous membrane includes the lung epithelial cells that are seeded and cultured on one side of the porous membrane, and the porous membrane includes the lung fibroblasts and the vascular endothelial cells that are seeded and cultured on the opposite side of the porous membrane,
wherein the lung epithelial cells are alveoli epithelial cells, and
wherein the first layer comprises one or more gas partial pressure sensors and the third layer comprises one or more pH sensors;
a step of adjusting a pressure of a perfusion gas for simulating respiration movement; and
a step of measuring pH by the pH sensor of the system and measuring a gas partial pressure by the gas partial pressure sensor.
2 . The microfluidic control method in the microfluidic system simulating a lung tissue according to claim 1 ,
wherein when the pH or the gas partial pressure measured in the step of measuring pH and gas partial pressure is different from pH or a gas partial pressure in a human lung tissue, the control method further comprises a step of adjusting an injection amount per hour of the gas and the medium-containing fluid injected into the microfluidic system simulating a lung tissue.
3 . The microfluidic control method in the microfluidic system simulating a lung tissue according to claim 1 ,
wherein the gas partial pressure is a partial oxygen pressure (pO 2 ).