Microfluidic systems and support module
This disclosure describes hardware for microfluidic chips and an associated support module for facilitating operation of one or more microfluidic chips. The microfluidic chips described herein are designed for supporting multiple different tissue types, including kidney tissue, liver tissue, adipose cells, and so forth. Chip geometry facilities fluid flow through one or more channels of the chip with a particular flow rate. For example, shear forces are reduced where needed to ensure proper flow rate of fluid in the channels. The chamber geometry and the geometry of the channels ensures that a desired amount of oxygen is delivered to sample cells or tissues in a controlled manner.
1 . A microfluidic chip, comprising:
a pump configured to pump a fluid media through a fluid circuit;
a cell chamber in the fluid circuit, the cell chamber supporting a plurality of cells, the cell chamber configured to receive the fluid media including oxygen for exposing the plurality of cells to the fluid media including the oxygen; and
a re-oxygenation chamber configured to add oxygen to the fluid media circulating in the fluid circuit,
wherein the cell chamber forms part of an insert that is removable from the re-oxygenation chamber and pump of the fluid circuit
wherein the cell chamber comprises a waterfall feature, the waterfall feature including a step from a first level of the cell chamber to a second level of the cell chamber.
2 . The microfluidic chip of claim 1 , further comprising:
a flow sensor configured to measure a flow rate of the fluid media in the fluid circuit; and
a controller in communication with the flow sensor and the pump, the controller configured to:
obtain data specifying a desired flow rate of the fluid media;
receive a signal specifying the flow rate measured by the flow sensor; and
generate, in response to receiving the signal specifying the flow rate, a pump control signal configured to cause the pump to pump the fluid media at the desired flow rate.
3 . The microfluidic chip of claim 1 , further comprising a valve, the valve being moveable to a first position that allows the fluid media to circulate in the fluid circuit through the cell chamber, and the valve being moveable to a second position that isolates the cell chamber from the fluid circuit to enable seeding of the cell chamber with the cells.
4 . The microfluidic chip of claim 1 , further comprising a sample carousel, the sample carousel comprising one or more sample chambers configured to store a portion of the fluid media.
5 . The microfluidic chip of claim 1 , wherein the re-oxygenation chamber comprises an oxygen permeable membrane for receiving oxygen from an environment outside of the fluid circuit.
6 . The microfluidic chip of claim 1 , further comprising a pH sensor configured to measure a pH of the fluid media.
7 . The microfluidic chip of claim 1 , wherein the fluid circuit comprises a resistive tubing configured to limit a flow rate in the fluid circuit.
8 . The microfluidic chip of claim 1 , further comprising:
a first oxygen concentration sensor configured to measure oxygen concentration in the fluid media at an input of the cell chamber; and
a second oxygen concentration sensor configured to measure the oxygen concentration in the fluid media at an output of the cell chamber.
9 . The microfluidic chip of claim 1 , further comprising an air inlet port configured to provide oxygen to the re-oxygenation chamber, and an air outlet port configured to remove air from the re-oxygenation chamber.
10 . The microfluidic chip of claim 1 , further comprising a second fluid circuit comprising a second cell chamber, the second fluid circuit configured to circulate second fluid media that is isolated from the fluid media of the fluid circuit.
11 . The microfluidic chip of claim 1 , further comprising a sampling port configured to enable removal of a portion of the fluid media from the fluid circuit.
12 . The microfluidic chip of claim 1 , wherein the cell chamber comprises a seed port for seeding the cell chamber and a waste port for removing waste cells from the cell chamber.
13 . The microfluidic chip of claim 1 , wherein the step is 2-3 mm in height.
14 . The microfluidic chip of claim 1 , wherein the insert includes a platform for perfusion of cells, for seeding cells, or both.
15 . The microfluidic chip of claim 14 , wherein the platform includes a permeable membrane configured to allow the fluid media to pass through and supports the plurality of cells.
16 . The microfluidic chip of claim 1 , wherein the insert includes a top cover configured to be coupled to a top of a substrate to seal the cell chamber.
17 . The microfluidic chip of claim 1 , wherein the insert includes a bottom cover configured to be coupled to a bottom layer to seal the cell chamber, the bottom cover including the cell chamber.
18 . The microfluidic chip of claim 1 , wherein the cell chamber includes a single compartment.
19 . The microfluidic chip of claim 1 , wherein the cell chamber includes a dual-compartment configuration including an apical side and a basal side.
20 . The microfluidic chip of claim 1 , wherein the cell chamber is a first cell chamber, the microfluidic chip further comprising a second cell chamber configured to receive the fluid media in sequence with the first cell chamber.
21 . The microfluidic chip of claim 1 , further comprising a transwell adapter for interfacing the cell chamber with a transwell.
22 . The microfluidic chip of claim 1 , wherein the cell chamber is part of an insert that is configured for seeding the plurality of cells, the microfluidic chip further comprising:
one or more clamps configured to secure the insert; and
a seeding cover configured to cover the insert.
23 . The microfluidic chip of claim 1 , further comprising a port for removal of the fluid media, the port comprising a valve enabling complete removal of fluid media from the fluid circuit.
24 . A support module, comprising:
a controller; and
a platform configured to receive the microfluidic chip of any of claims 1-12 , the platform configured to couple the microfluidic chip to the controller for communicating data between the controller and the pump of the microfluidic chip.
25 . The support module of claim 24 , further comprising a user interface configured to enable a user to input control parameters for controlling operation of the microfluidic chip, the control parameters comprising one or more of a pump speed, an oxygen concentration in the cell chamber, and a pump speed of the pump.
26 . The support module of claim 24 , further comprising a memory port configured to receive a memory device, the memory configured to store one or more configuration parameters for operation of the microfluidic chip and data received from the microfluidic chip.
27 . The support module of claim 24 , wherein the controller is configured to:
receive identifier data from the microfluidic chip;
obtain one or more control parameters that are associated with the identifier; and
automatically control operation of the microfluidic chip in accordance with the one or more control parameters.
28 . The support module of claim 24 , wherein the controller is configured to control operation of a plurality of instances of the microfluidic chip in parallel.
29 . The support module of claim 24 , further comprising a mechanical device configured to interface with a sampling carousel on the microfluidic chip, wherein the mechanical device is configured to control a position of the sampling carousel during operation of the microfluidic chip.
30 . The support module of claim 24 , further comprising:
a port configured to interface a sensor with the microfluidic chip for detecting at least one of an oxygen level in the fluid media, a pH of the fluid media, or both.