Gene for boosting genetic transformation efficiency of wheat and use thereof
Disclosed is a gene for boosting genetic transformation efficiency of wheat and use thereof, which belongs to the field of plant genetic engineering technology. The use of a TaHRF1 gene and an encoded protein thereof can promote integration of an exogenous nucleic acid molecule into a genome of a plant of interest, and boost efficiency of the integration of the nucleic acid molecule, and the transformed plant grows and develops normally. The plant of interest includes, but is not limited to, monocotyledonous plants such as wheat. The present disclosure provides a new and important genetic resource for overcoming the genotype dependence in genetic transformation of wheat.
1 . A method for boosting genetic transformation efficiency of wheat, comprising:
transforming a TaHRF1 gene and a target nucleic acid molecule into a target plant,
wherein the TaHRF1 gene is the nucleic acid molecule of SEQ ID NO: 1 or
the nucleic acid molecule encoding the amino acid sequence of SEQ ID NO: 2, excluding SEQ ID NO:1;
wherein the TaHRF1 gene and the target nucleic acid molecule are transferred to the wheat by a pc186 expression vector;
wherein a variety of the wheat is Liangxing 66 or Aifeng 3.
2 . The method of claim 1 , wherein the protein encoded by the TaHRF1 gene is the protein as shown in any one of (A1) or (A2) below:
(A1) the protein consisting of the amino acid sequence of SEQ ID NO: 2;
(A2) a fusion protein obtained by attaching a protein tag to the N-terminus and/or the C-terminus of the protein defined in (A1).