Mass tags for lipid analyses
The present disclosure provides compositions comprising novel mass tags. The disclosure also provides methods utilizing mass tags, including methods of quantifying one or more individual lipids from a composition of lipids, methods of identifying a double bond position of an individual lipid, methods of identifying a biological correlation of an individual lipid, and methods of preparing a lipid derivative.
1 . A composition comprising a mass tag, wherein the mass tag has a structure of
wherein n is between 0 and 10.
2 . A composition comprising a mass tag, wherein the mass tag has a structure selected from the group consisting of:
wherein n is between 0 and 10.
3 . The composition of claim 2 , wherein the mass tag has a structure of
4 . The composition of claim 2 , wherein the mass tag has a structure of
5 . The composition of claim 2 , wherein the mass tag has a structure of
6 . A method of quantifying one or more individual lipids from a composition of lipids, said method comprising the steps of:
combining the composition of lipids with one or more mass tags to form a tagged lipid composition,
performing mass spectrometry on the tagged lipid composition to identify the one or more individual lipids, and
quantifying the one or more individual lipids,
wherein the mass tag is present as the composition of claim 2 .
7 . The method of claim 6 , wherein the mass tag has a structure of
wherein n is between 0 and 10.
8 . The method of claim 6 , wherein the composition of lipids comprises one or more unsaturated lipids.
9 . The method of claim 6 , wherein the composition of lipids is blood.
10 . The method of claim 6 , wherein the composition of lipids is plasma.
11 . The method of claim 6 , wherein the method does not require a lipid internal standard.
12 . The method of claim 6 , wherein the mass spectrometry comprises tandem mass spectrometry.
13 . The method of claim 6 , wherein the method comprises a step of identifying a double bond position of the one or more individual lipids.
14 . The method of claim 13 , wherein the mass tag has a structure of
wherein n is between 0 and 10.
15 . The method of claim 13 , wherein the double bond position is a carbon-carbon double bond position.
16 . The method of claim 13 , wherein the composition of lipids comprises one or more unsaturated lipids.
17 . The method of claim 13 , wherein the composition of lipids is blood or plasma.
18 . The method of claim 13 , wherein the method does not require a lipid internal standard.
19 . The method of claim 13 , wherein the mass spectrometry comprises tandem mass spectrometry.