IP Library › Granted Patent US 12673022
Granted Patent B2
US 12673022 · App. 17/298,460 · Granted Jul 7, 2026

Drug delivery system using solution

Inventors: Akitsu Hotta (Kyoto, JP); Naoko Ishihara (Kyoto, JP); Ryuichi Nishigaki (Kanagawa, JP); Masaki Seto (Kanagawa, JP)
Assignees: Kyoto University; Takeda Pharmaceutical Company Limited
A61K9/08A61K31/7088A61K38/465A61K47/10A61K47/12A61K47/16A61K47/18A61K47/183A61K47/22A61K47/24A61K47/26A61K48/00
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Quick Facts
Patent No.
US 12673022
App. No.
17/298,460
Granted
Jul 7, 2026
Kind
B2
Abstract

Provided are methods for transducing a molecule(s) of interest into a cell, comprising a step of contacting the cell with the molecule(s) of interest and a solution for transduction, the solution for transduction containing: at least one of the following (A1) to (A5); and (B) a salt: (A1) a compound represented by formula (I) excluding a predetermined compound, or a salt thereof; (A2) a compound represented by formula (II), or a salt thereof; (A3) a nucleic-acid base or the like, or a salt thereof; (A4) a compound represented by formula (III), or a salt thereof, excluding malic acid; and (A5) at least one selected from the group consisting of creatinine, hydroxyproline, 1,3-butanediol, trientine, D-cellobiose, 1,3-dimethylurea, pantolactone and trimethadione, or a salt thereof.

Claims (15)

1 . A pharmaceutical composition comprising:

a solution for transduction comprising:

at least one compound selected from the group consisting of 1-methyl-L-histidine, N-carbamyl-L-aspartic acid, 2-deoxy-D-glucose-6-phosphate, 6-phospho-D-gluconic acid, and a salt thereof,

(B) a salt, and

a Cas protein and/or gRNA.

2 . A method for transducing a molecule(s) of interest into a cell, comprising a step of contacting the cell with the molecule(s) of interest and a solution for transduction, wherein

the solution for transduction comprises: at least one compound selected from the group consisting of 1-methyl-L-histidine, N-carbamyl-L-aspartic acid, 2-deoxy-D-glucose-6-phosphate, 6-phospho-D-gluconic acid, and a salt thereof; and (B) a salt, and wherein the molecule(s) of interest is a Cas protein and/or gRNA.

3 . The method according to claim 2 , wherein the (B) salt is at least one selected from the group consisting of sodium chloride, magnesium chloride and potassium chloride.

4 . The method according to claim 2 , wherein the cell is present in a living body.

5 . The method according to claim 2 , wherein the cell is a muscle cell.

6 . A method for producing a cell having a molecule(s) of interest transduced therein, comprising a step of contacting the cell with a molecule(s) of interest and a solution for transduction, wherein

the solution for transduction comprises: at least one compound selected from the group consisting of 1-methyl-L-histidine, N-carbamyl-L-aspartic acid, 2-deoxy-D-glucose-6-phosphate, 6-phospho-D-gluconic acid, and a salt thereof; and (B) a salt, and wherein the molecule(s) of interest is a Cas protein and/or gRNA.

7 . The method according to claim 6 , wherein the (B) salt is at least one selected from the group consisting of sodium chloride, magnesium chloride and potassium chloride.

8 . The method according to claim 6 , wherein the cell is present in a living body.

9 . The method according to claim 6 , wherein the cell is a muscle cell.