Pegylated liposomes and uses thereof
Provided herein are PEGylated liposomes, and methods of making and using thereof. The PEGylated liposomes comprise DSPE-peg2000 and nosiheptide in a molar ratio of 3:1. Compositions and methods which is related to making the PEGylated liposomes and using the PEGylated liposomes for preventing or treating bacterial infection are also provided.
1 . A liposome comprising Distearoylphosphatidylethanolamine-N-[methoxy(poly(ethyleneglycol))-2000] (DSPE-peg2000) and nosiheptide, wherein the molar ratio of DSPE-peg2000:nosiheptide is 3:1.
2 . The liposome of claim 1 , which is about 130 nm to 200 nm in diameter.
3 . The liposome of claim 1 , wherein a polydispersity index of the liposome is less than 0.3.
4 . A pharmaceutical composition comprising the liposome of claim 1 and a pharmaceutically acceptable carrier.
5 . A method for treating bacterial infection in a subject in need thereof comprising: administering to said subject a pharmaceutically effective amount of the composition of claim 4 .
6 . The method of claim 5 , wherein the subject is a human.
7 . The method of claim 5 , wherein the bacterial infection is caused by at least one strain of gram-positive bacteria.
8 . The method of claim 7 , wherein the strain of gram-positive bacteria is selected from the group consisting of Vancomycin Resistant Staphylococcus aureus 01, Staphylococcus aureus ATCC29213, and Staphylococcus aureus ATCC33591.
9 . The method of claim 5 , wherein the composition is administered via intravenous injection.
10 . A method of preparing the liposome of claim 1 comprising the steps of: a) dissolving DSPE-peg2000 in an organic solvent; b) dissolving nosiheptide in another organic solvent which is miscible with the solvent in step a; c) mixing the DSPE-peg2000 solution from step a and nosiheptide solution from step b with the molar ratio of DSPE-peg2000:nosiheptide equaling to 3:1; d) removing the solvent to form a thin film; e) hydrating the thin film in an aqueous medium to form multilamellar vesicles (MLVs) liposomes; and f) sonicating the MLVs solution whereby liposomes consisting of single unilamellar vesicles are obtained.
11 . The method of claim 10 , wherein the organic solvent in step a is methylene-chloride and the organic solvent in step b is dimethylformamide.
12 . The method of claim 10 , wherein the aqueous medium in step e is phosphate buffer saline (PBS).