Antimycotic methoxyflavone and fluconazole combination for inhibiting cryptococcus
The present invention relates to methods and means for inhibiting or preventing the growth of fungal cells with at least one flavone selected from the group consisting of 5-methoxyflavone, 2′-methoxyflavone, 7-methoxyflavone, 3′,4′,5,7-tetrametoxyflavone, 3′,4′,5′,5,6,7-hexymethoxyflavone, 7,8-benzoflavone and 5,6-benzoflavone for inhibiting or preventing the growth of a fungal cell, wherein said flavone is used in combination with at least one further antimycotic compound selected from the group of azoles, both in an amount to exhibit a synergistic effect compared to the separate use of said flavone and said antimycotic compound.
1 . A method of for inhibiting or preventing the growth of a fungal cell selected from the group consisting of Cryptococcus fungi , the method comprising contacting fungal cells with 5-methoxyflavone, in combination with fluconazole, both in an amount to exhibit a synergistic effect compared to the separate use of said 5-methoxyflavone and said fluconazole.
2 . The method according to claim 1 , wherein the fungal cell is a biofilm forming cell.
3 . The method according to claim 1 , wherein the fungal cell is selected from the group consisting of Cryptococcus neoformans and Cryptococcus gattii.
4 . The method according to claim 1 , wherein the 5-methoxyflavone is applied to a plant or parts thereof.
5 . A method for treating a fungal infection in a human or animal caused by a fungus selected from the group consisting of Cryptococcus fungi , the method comprising administering to the human or animal of 5-methoxyflavone, wherein the 5-methoxyflavone is administered to the human or animal together or subsequently with fluconazole-2′ methoxyflavone.
6 . The method according to claim 5 , wherein the 5-methoxyflavone and fluconazole are administered orally, topically, or intravenously.
7 . A method for inhibiting or preventing the growth of a fungal cell comprising the step of contacting fungal cells with a composition comprising 5-methoxyflavone and fluconazole, both in an amount to exhibit a synergistic effect compared to the separate use of said 5-methoxyflavone and said fluconazole, with the proviso that the fungal cells are not C. albicans.
8 . The method according to claim 4 , wherein the plant or parts are selected from fruits or leaves.
9 . The method according to claim 5 , wherein the animal is a mammal.
10 . The method according to claim 7 , wherein the method is for treating a fungal infection in a human or animal caused by a fungus, the method comprising administering the composition to the human or animal.
11 . The method according to claim 10 , wherein the composition is administered orally, topically, or intravenously.
12 . The method according to claim 10 , wherein the animal is a mammal.