DNA vaccine for SARS-COV-2
Provided is DNA that: encodes a coronavirus (SARS COV-2) spike protein or a fragment thereof; and has been optimized to partially or fully exhibit a codon included in a DNA sequence.
1 . A DNA encoding a coronavirus SARS COV-2 spike protein or a fragment thereof, comprising a nucleotide sequence having 950% or more identity with a nucleotide sequence shown by SEQ ID NO: 3, 12, 14, 16 or 18.
2 . The DNA according to claim 1 , having a nucleotide sequence shown by SEQ ID NO: 3, 12, 14, 16 or 18.
3 . A nucleic acid construct containing a promoter that functions in a human and the DNA according to claim 1 operably linked to the promoter.
4 . The nucleic acid construct according to claim 3 , further containing a transcription termination sequence operably linked to the DNA.
5 . The nucleic acid construct according to claim 3 , which is incorporated into a vector.
6 . The nucleic acid construct according to claim 5 , which is incorporated into a vector selected from the group consisting of a plasmid vector, a phage vector and a viral vector.
7 . A pharmaceutical composition comprising the nucleic acid construct according to claim 3 .
8 . The pharmaceutical composition according to claim 7 , further comprising an adjuvant.
9 . The pharmaceutical composition according to claim 7 , which is a vaccine for coronavirus infection.