Heterologous proteins with axonemal proteins
Provided herein are compositions and methods for the production of heterologous proteins in a cell including axonemes. The cells include a nucleic acid encoding a fusion protein expressed in the axoneme. The fusion protein includes an axonemal protein linked to a heterologous protein, and the fusion protein provides axonemal function to the cell.
1 . An algal cell comprising: a nucleic acid encoding a fusion protein, wherein the fusion protein comprises an axonemal protein linked to a heterologous protein through an enzyme cleavable linker, wherein said axonemal protein is FAP20, and wherein the heterologous protein is an enzyme.
2 . The algal cell of claim 1 , wherein the enzyme is selected from retro-aldolase, Kemp-eliminase HG3.17 (KE), Aspergillus niger monoamine oxidase variant D5 (MAO), NADH oxidase (NOX), and beta-lactamase.
3 . A plurality of algal cells wherein each algal cell comprises: a nucleic acid encoding a fusion protein, wherein the fusion protein comprises an axonemal protein linked to a heterologous protein through an enzyme cleavable linker, wherein said axonemal protein is FAP20, and wherein the heterologous protein is an enzyme.
4 . The plurality of algal cell of claim 3 , wherein the enzyme is selected from retro-aldolase, Kemp-eliminase HG3.17 (KE), Aspergillus niger monoamine oxidase variant D5 (MAO), NADH oxidase (NOX), and beta-lactamase.
5 . A recombinant protein comprising:
a. an algal axonemal protein, and
b. a heterologous protein;
wherein the axonemal protein is linked to the heterologous protein through an enzyme cleavable linker; wherein said axonemal protein is FAP20; and wherein the heterologous protein is an enzyme.
6 . The recombinant protein of claim 5 , wherein the enzyme is selected from retro-aldolase, Kemp-eliminase HG3.1 7 (KE), Aspergillus niger monoamine oxidase variant D5 (MAO), NADH oxidase (NOX), and beta-lactamase.
7 . An isolated nucleic acid encoding the recombinant protein of claim 5 .