IP Library › Granted Patent US 12673984
Granted Patent B2
US 12673984 · App. 16/467,398 · Granted Jul 7, 2026

VHH-containing heavy chain antibody and production thereof

Inventors: Friedrich Nolte (Hamburg, DE); Thomas Eden (Hamburg, DE); Janusz Wesolowski (Hamburg, DE); Stephan Menzel (Hamburg, DE)
Assignee: Universitätsklinikum Hamburg-Eppendorf
C07K16/18A01K67/0275A01K67/0278C12N15/64A01K2217/072A01K2227/105A01K2267/01C07K2317/22C07K2317/569C12N2800/204C12N2800/24C12N2800/90
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Quick Facts
Patent No.
US 12673984
App. No.
16/467,398
Granted
Jul 7, 2026
Kind
B2
Abstract

The present invention contemplates isolated polynucleotide for the production of a VHH-containing heavy chain antibody in a mammal and vectors comprising said isolated polynucleotide. Moreover, the invention relates to a transgenic mammal comprising the vector for the production of a VHH-containing heavy chain antibody. Further, the invention relates to VHH-containing heavy chain antibodies as well as methods for the production and cloning of VHH-containing heavy chain antibodies.

Claims (9)

1 . An isolated polynucleotide for the production of a VHH-containing heavy chain antibody in a mammal, wherein the polynucleotide comprises SEQ ID NO: 30.

2 . A transgenic mouse or rat comprising the isolated polynucleotide of claim 1 .

3 . The transgenic mouse or rat of claim 2 , wherein the transgenic mouse or rat expresses the VHH-containing heavy chain antibody as a membrane bound version.

4 . A method for the production of a VHH-containing heavy chain antibody in a non-human mammal, the method comprising the step of expressing the polynucleotide of SEQ ID NO:30 in said non-human mammal.

5 . A method for cloning a VHH-containing heavy chain antibody from a transgenic non-human mammal expressing the polynucleotide of SEQ ID NO:30, the method comprising the steps of:

(i) immunizing said transgenic non-human mammal expressing the polynucleotide of SEQ ID NO: 30, and immortalizing B cells expressing a heterologous VHH-containing heavy chain antibody from said transgenic non-human mammal, or

(ii) immunizing said transgenic non-human mammal expressing the polynucleotide of SEQ ID NO: 30, and obtaining VHH-coding sequences for the heterologous VHH-containing heavy chain antibody from B cells of said transgenic non-human mammal,

wherein the heterologous VHH-containing heavy chain antibody is encoded by the isolated polynucleotide of claim 1 .

6 . The transgenic mouse or rat of claim 2 , wherein the transgenic mouse or rat expresses the VHH-containing heavy chain antibody as a soluble version.