Well for cultivating biological material

1 . A multi-well plate comprising a plurality of wells for cultivating biological material, each well of the plurality of wells comprising:

a vertical side wall;

a top opening;

a bottom area comprising a first internal edge;

a second internal edge arranged on top of the first internal edge;

an upper cylinder extending from the top opening to the second internal edge;

a cylindrical pipetting notch; and

a barrier wall, formed by the first internal edge and the second internal edge, located between the pipetting notch and the bottom area,

wherein the bottom area extends from the second internal edge to a bottom of the well,

wherein the first and second internal edge are annular with a rectangular section and are partially circumferential along a longitudinal axis of the well,

wherein the second internal edge is arranged on top of the first internal edge on a side of the first internal edge, which is opposite to the side with which the first internal edge sits on the bottom of the well,

wherein the second internal edge has a larger internal diameter than the first internal edge and is higher than the first internal edge,

wherein the first and second internal edges form the barrier wall,

wherein the second internal edge does not form the vertical side wall of the well thereby the second internal edge has larger internal diameter than the vertical side wall and/or is a length apart along the horizontal axis from the vertical side wall,

wherein each well of the plurality of wells comprises a microwell-structure, the microwell-structure comprising a plurality of microwells, and

wherein the microwell-structure is made of stamped hydrogel in the bottom area of the well.

2 . The multi-well plate according to claim 1 , wherein each well of the plurality of wells is configured to receive the stamped hydrogel as a hydrogel drop in the bottom area, wherein the first internal edge is configured to force the hydrogel drop to develop a dome surface after a sufficient volume of the hydrogel drop has been placed in the bottom area of the well.

3 . The multi-well plate according to claim 1 , wherein at least a part of the first internal edge and/or the second internal edge is essentially circumferential and/or essentially ring-like.

4 . The multi-well plate according to claim 3 , wherein the first internal edge is located between a bottom of the well and the second internal edge.

5 . The multi-well plate according to claim 3 , wherein at least a part of each well of the plurality of wells is essentially cylindrical, wherein at least a part of the upper cylinder and/or the bottom area is essentially cylindrical with an essentially round section.

6 . The multi-well plate according to claim 5 , wherein an internal diameter of the bottom area is smaller than an internal diameter of the upper cylinder.

7 . The multi-well plate according to claim 5 , wherein the upper cylinder and the bottom area and the first internal edge are molded into one and the same workpiece, wherein the second internal edge is also molded into the same workpiece, wherein the workpiece is made from plastic and/or wherein a wall thickness of the workpiece is essentially constant throughout the entire well.

8 . The multi-well plate according to claim 5 , wherein the pipetting notch is essentially circular cylindrical, wherein a longitudinal axis (L. 3 ) of the pipetting notch and a longitudinal axis (L. 2 ) of the upper cylinder are parallel, wherein a distance between the longitudinal axis (L. 3 ) of the pipetting notch and the longitudinal axis (L. 2 ) of the upper cylinder is smaller than a sum of an inner radius of the upper cylinder and an inner radius of the pipetting notch, such that the upper cylinder and the pipetting notch overlap, at least along a fraction of the depth of the well, such that an opening is present between the pipetting notch and the upper cylinder.

9 . The multi-well plate according to claim 8 , wherein a height of the pipetting notch equals the sum of a height of the upper cylinder and a height of the bottom area, wherein a height (h. 15 ) of the barrier wall equals the height (h. 14 ) of the bottom area.

10 . The multi-well plate according to claim 8 , wherein the multi-well plate comprises a main body, wherein the wells and/or all hollow cylinders and/or all internal edges and/or all pipetting notches are molded into the main body, wherein a wall thickness of the main body is essentially constant throughout the main body.

11 . A method for manufacturing the multi-well plate according to claim 10 , comprising:

forming a main body comprising the multitude of the wells, wherein the main body is formed by means of injection molding,

injecting a hydrogel drop into the bottom area of each well, and

stamping a microwell-structure into each hydrogel drop.

12 . The multi-well plate according to claim 1 , wherein each well of the plurality of wells comprises a first hollow cylinder, wherein the first hollow cylinder comprises a first top rim, wherein at least a part of the first top rim forms the first internal edge, wherein the first hollow cylinder extends from a bottom of the well towards the top opening, wherein the first hollow cylinder is placed concentrically inside the bottom area wherein an external diameter of the first hollow cylinder equals the internal diameter of the bottom area.

13 . The multi-well plate according to claim 12 , wherein each well of the plurality of wells comprises a second hollow cylinder, wherein the second hollow cylinder comprises a second top rim, wherein at least a part of the second top rim forms the second internal edge.