Miniaturized centrifugal bioreactor and rotor system
A centrifugal bioreactor includes a turntable assembly, an electric motor coupled to the turntable assembly, and a replaceable rotor assembly coupled to the turntable assembly. The replaceable rotor assembly includes a plurality of reaction chambers that house cell cultures. A fresh medium tank holds fresh medium for the cell cultures, a spent medium tank holds a first portion of spent medium produced by the cell cultures, and an inoculation tank combines the fresh medium from the fresh medium tank and a second portion of the spent medium. A gas exchanger exposes the fresh medium and the second portion of the spent medium to gas. Additionally, a first pump supplies the fresh medium and the second portion of the spent medium to the plurality of the reaction chambers, and a second pump discard the first portion of the spent medium to the spent medium tank.
1 . A centrifugal bioreactor comprising:
a turntable assembly;
an electric motor coupled to the turntable assembly;
a replaceable rotor assembly coupled to the turntable assembly, the replaceable rotor assembly including a central disc that forms (i) a plurality of reaction chambers configured to house cell cultures and (ii) conduits configured to provide fluid routing to and from the plurality of reaction chambers, respectively;
a fresh medium tank configured to hold fresh medium for the cell cultures;
a spent medium tank configured to hold a spent medium produced by the cell cultures;
an inoculation tank configured to combine the fresh medium with a portion of the spent medium;
a gas exchanger configured to expose the fresh medium and the portion of the spent medium to gas;
a first pump fluidly connected to the fresh medium tank, the inoculation tank, and the replaceable rotor assembly, wherein individual reaction chambers of the plurality of reaction chambers are in direct fluid connection with the first pump; and
a second pump fluidly connected to the replaceable rotor assembly and the spent medium tank.
2 . The centrifugal bioreactor of claim 1 , further comprising:
a supply line configured to supply the fresh medium and the portion of the spent medium to the plurality of reaction chambers; and
a spent medium line configured to receive the spent medium from the plurality of reaction chambers.
3 . The centrifugal bioreactor of claim 1 , wherein individual reaction chambers of the plurality of reaction chambers include:
an inlet; and
an outlet located closer to a center of the replaceable rotor assembly than the inlet.
4 . The centrifugal bioreactor of claim 3 , wherein individual reaction chambers of the plurality of reaction chambers are defined at least in part by:
a longitudinal axis extending through the inlet and the outlet;
a first section fluidly connected to the inlet, the first section including sidewalls that extend in a direction away from the longitudinal axis; and
a second section fluidly connected to the first section and the outlet, the second section including sidewalls that extend in a direction towards the longitudinal axis.
5 . The centrifugal bioreactor of claim 1 , further comprising a housing having a first compartment and a second compartment wherein:
the fresh medium tank, the spent medium tank, the inoculation tank, the gas exchanger, the first pump, and the second pump reside in the first compartment; and
the turntable assembly and the replaceable rotor assembly reside in the second compartment.
6 . The centrifugal bioreactor of claim 1 , wherein:
the replaceable rotor assembly includes a top plate and a bottom plate;
the top plate couples to the central disc to enclose the plurality of reaction chambers on a first side; and
the bottom plate couples to the central disc to enclose the plurality of reaction chambers on a second side that is opposite the first side.
7 . The centrifugal bioreactor of claim 6 , wherein the central disc, the top plate, and the bottom plate have a central axis of rotation.
8 . The centrifugal bioreactor of claim 1 , wherein the central disc encloses the plurality of reaction chambers.
9 . The centrifugal bioreactor of claim 1 , wherein the plurality of reaction chambers are substantially equal in size.
10 . A centrifugal bioreactor comprising:
a rotor assembly including:
one or more layers having a common diameter,
a plurality of reaction chambers formed by the one or more layers, the plurality of reaction chambers configured to house cell cultures, and
conduits formed by the one or more layers and fluidly connected to the plurality of reaction chambers, the conduits providing fluid routing to and from the plurality of reaction chambers, respectively;
a fresh medium tank configured to hold fresh medium for the cell cultures;
a spent medium tank configured to hold spent medium produced by the cell cultures;
an inoculation tank configured to combine the fresh medium from the fresh medium tank and a portion of the spent medium;
a gas exchanger configured to expose the fresh medium and the portion of the spent medium to gas;
a pump configured to supply the fresh medium and the portion of the spent medium; and
a manifold connected to the pump to supply the fresh medium and the portion of the spent medium to individual reaction chambers of the plurality of reaction chambers.
11 . The centrifugal bioreactor of claim 10 , wherein:
the one or more layers include a top plate, a central disc, and a bottom plate;
the top plate encloses the plurality of reaction chambers on a first side;
the central disc encloses a perimeter of the plurality of reaction chambers; and
the bottom plate encloses the plurality of reaction chambers on a second side.
12 . The centrifugal bioreactor of claim 10 , wherein the one or more layers enclose the plurality of reaction chambers.