Nanozyme material and preparation method thereof, and detection method of lead (II) ion
A nanozyme material and a preparation method thereof are provided. In the preparation method, Prussian blue (PB) is used to regulate the peroxidase activity of Fe,NA/CDs, and then gold nanoparticles are synthesized with Fe,NA/CDs as a reducing agent and a stabilizing agent. The nanozyme material has both a peroxidase activity and a Raman enhancement effect, which can be used for nanozyme-based surface-enhanced Raman spectroscopy (SERS). An embodiment of the present disclosure also provides a method for rapidly detection of a Pb 2+ ion, which is based on a color change or an additional Raman SERS signal generation caused by nanozyme oxidation. Such sensing signals (colorimetric and SERS analysis) are derived from a catalytic oxidation reaction of ABTS or TMB, while the presence of Pb 2+ specifically inhibits the oxidation, such that the rapid detection of Pb 2+ can be achieved.
1 . A preparation method of a nanozyme material, wherein the nanozyme material is Fe, norepinephrine (NE)/CDs-Prussian blue (PB) @AuNPs, and the nanozyme material comprises iron-doped norepinephrine carbon dots Fe (NE)/CDs, a Prussian blue (PB) loaded on the iron-doped norepinephrine carbon dots Fe (NE)/CDs, and AuNPs distributed on a surface of the iron-doped norepinephrine carbon dots Fe, (NE)/CDs, and wherein the method comprises:
mixing
Fe NE/CDs-PB, H 2 O 2 , sodium citrate and HAuCl 4 in water to obtain a mixed solution, wherein Fe,NE/CDs-PB, H 2 O 2 , and sodium citrate are present in a mass ratio of 1:(0.02-0.2):(1-3), H 2 O 2 is in a solution concentration of 30 mmol/L to 70 mmol/L, based on a mass of Fe,NE/CDs-PB an amount of 2 L to 4 L of water is added per g of Fe,NE/CDs-PB, and a concentration of HAuCl 4 in the mixed solution is 5 mg/mL to 20 mg/mL; and stirring the mixed solution at room temperature for 5 min to 10 min to obtain Fe NE/CDs-PB@AuNPs.
2 . The preparation method according to claim 1 , wherein a preparation method of the Fe, (NE)/CDs-PB comprises:
mixing a solution of the PB in hydrochloric acid with the Fe, (NE)/CDs to obtain the Fe, (NE)/CDs-PB.
3 . The preparation method according to claim 2 , wherein a mass ratio of the Fe, (NE)/CDs to the PB is 1:(20-60).
4 . The preparation method according to claim 2 , wherein a preparation method of the Fe (NE)/CDs comprises:
mixing norepinephrine bitartrate, ethylenediamine (EDA), and iron chloride to obtain a resulting mixture, and subjecting the resulting mixture to a second reaction at 160° C. to 200° C.
5 . The preparation method according to claim 4 , wherein the norepinephrine bitartrate, the EDA, and the iron chloride are in a mass ratio of 1:(0.2-0.5):(0.03-0.15).