Bacterial hosts for recombinant protein expression
The present invention provides recombinant gram-negative host cells that do not degrade protease-sensitive recombinant proteins yet grow to high cell density, methods for the use of these host cells to produce high-quality recombinant proteins, including antibodies and antibody fragments, at high yield, as well as compositions and methods relating to periplasmic expression of recombinant proteins or polypeptides of interest in host cells.
1 . A recombinant gram-negative Pseudomonas fluorescens host cell for recombinant protein expression, wherein the host cell is:
(a) deficient in a first protease activity, wherein the first protease activity is tail-specific protease activity, wherein the deficient first protease activity results from a mutation in a gene encoding a Prc1 tail-specific protease having the amino acid sequence set forth as SEQ ID NO: 33, a Prc1 tail-specific protease related protein having an amino acid sequence at least 90% similar to SEQ ID NO: 33, a Prc2 tail-specific protease having the amino acid sequence set forth as SEQ ID NO: 35, or a Prc2 tail-specific protease related protein having an amino acid sequence at least 90% similar to SEQ ID NO: 35; and,
(b) deficient in a second protease activity, wherein the second protease activity is murein DD-endopeptidase activity, wherein the deficient second protease activity results from a mutation in at least one gene encoding a murein DD-endopeptidase; and wherein the host cell further:
(c) is deficient in at least one additional protease activity, wherein the deficient additional protease activity results from a mutation in at least one gene encoding an additional protease, wherein the additional protease is different from the proteases of (a) and (b);
(d) is deficient in one or more autolytic factor activity, wherein the deficient autolytic factor activity results from a mutation in at least one gene encoding an autolytic factor;
(e) overexpresses one or more inactivated protease;
(f) overexpresses one or more folding modulator; or
(g) any combination of (c), (d), (e) and (f).
2 . The recombinant gram-negative Pseudomonas fluorescens host cell of claim 1 , wherein the deficient murein DD-endopeptidase activity results from a mutation in a gene encoding a MepM1 murein DD-endopeptidase having the amino acid sequence set forth as SEQ ID NO: 1, or a murein DD-endopeptidase related protein having an amino acid sequence at least 30% similar to SEQ ID NO: 1.
3 . The recombinant gram-negative Pseudomonas fluorescens host cell of claim 1 , wherein: the host cell of (c) is deficient in 1 to 10 different additional protease activities; the host cell of (d) is deficient in 1-5 different autolytic factor activities; the host cell of (e) overexpresses 1 to 10 different inactivated proteases, wherein each inactivated protease is different; the host cell of (f) overexpresses 1-10 different folding modulators; or any combination thereof.
4 . The recombinant gram-negative Pseudomonas fluorescens host cell of claim 1 , wherein:
the one or more deficient additional protease activity of (c) results from a mutation of a gene encoding a serralysin precursor having the amino acid sequence set forth as SEQ ID NO: 9, a serralysin precursor related protein having at least 60% similarity or at least 60% identity to the amino acid sequence set forth as SEQ ID NO: 9, a serralysin precursor having the amino acid sequence set forth as SEQ ID NO: 47, or a serralysin precursor related protein having at least 60% similarity or at least 60% identity to the amino acid sequence set forth as SEQ ID NO: 47,
the one or more deficient autolytic factor activity of (d) results from a mutation of a gene encoding an autolytic factor independently selected from: a linear gramicidin synthase subunit D, and a hemolysin precursor; the one or more inactivated protease of (e) is a mutant periplasmic serine endoprotease; and
the one or more folding modulator of (f) is a disulfide isomerase.
5 . The recombinant gram-negative Pseudomonas fluorescens host cell of claim 1 , wherein the mutation in (a) or (b) is in a coding sequence or noncoding sequence of the corresponding gene, and wherein the mutation is independently selected from: (i) a complete gene deletion, (ii) a partial gene deletion, (iii) a missense mutation, (iv) a nonsense mutation, (v) a frameshift mutation, (vi) an insertion, and (vii) any combination of (ii), (iii), (iv), (v) and (vi).
6 . The recombinant gram-negative Pseudomonas fluorescens host cell of claim 5 , wherein the missense mutation of (iii) results in a conservative or non-conservative amino acid substitution.
7 . The recombinant gram-negative Pseudomonas fluorescens host cell of claim 5 , wherein the noncoding sequence is a regulatory sequence.
8 . The recombinant gram-negative Pseudomonas fluorescens host cell of claim 1 , wherein the gram-negative bacterial host cell further comprises a functional protease activity, wherein the functional protease activity is the activity of: a MepS1 having the amino acid sequence set forth as SEQ ID NO: 5; or a MepS1 related protein having at least 50% sequence similarity to the P. fluorescens MepS1 protease amino acid sequence set forth as SEQ ID NO: 5.
9 . The recombinant gram-negative Pseudomonas fluorescens host cell of claim 1 , wherein the gram-negative bacterial host cell further comprises a functional protease activity, wherein the functional protease activity is: a MepS2 having the amino acid sequence set forth as SEQ ID NO: 7; or a MepS2 related protein having at least 50% sequence similarity to the P. fluorescens MepS2 protease amino acid sequence set forth as SEQ ID NO: 7.
10 . The recombinant gram-negative Pseudomonas fluorescens host cell of claim 1 , wherein the deficient first protease activity results from a mutation of a coding sequence and/or noncoding sequence.
11 . The recombinant gram-negative Pseudomonas fluorescens host cell of claim 1 , wherein the second protease activity is deficient due to a mutation that results in a conservative or non-conservative substitution in an active site amino acid or an allosteric site amino acid of a protease having the second protease activity.
12 . The recombinant gram-negative Pseudomonas fluorescens host cell of claim 1 , wherein the deficient second protease activity results from at least one mutation of the second protease gene, wherein the mutation results in a disruption of the amino acid sequence at a position corresponding to: (i) any one or more of residues 134 to 145 of SEQ ID NO: 1; (ii) any one or more of residues 319 to 411 of SEQ ID NO: 1; (iii) any or more of residues 361 to 378 of SEQ ID NO: 1; (iv) any one or more residue selected from 248, 319, 330, 332, 334, 337, 378, 410, and 411 of SEQ ID NO: 1; or any combination of (i), (ii), (iii), and (iv).
13 . The recombinant gram-negative Pseudomonas fluorescens host cell of claim 1 , wherein the deficient second protease activity results from a gene mutation that results in an amino acid substitution of SEQ ID NO: 1 selected from: Y248stop, G332S, D334N, A337T, H411Y, P410L, and any conservative or non-conservative amino acid substitution of any one of R319, H330, D334, H378, and H411.
14 . The recombinant gram-negative Pseudomonas fluorescens host cell of claim 1 , wherein the host cell is capable of high-density cell growth in culture.
15 . The recombinant gram-negative Pseudomonas fluorescens host cell of claim 1 , further comprising at least one expression construct, each expression construct comprising at least one nucleic acid sequence encoding a recombinant protein of interest.
16 . The recombinant gram-negative Pseudomonas fluorescens host cell of claim 15 , wherein the recombinant protein of interest is selected from: an antibody, antibody fragment, or derivative of an antibody or antibody fragment.
17 . The recombinant gram-negative Pseudomonas fluorescens host cell of claim 16 , wherein the antibody, antibody fragment, or derivative thereof is selected from: a monoclonal antibody; a complementarity determining region (CDR) fragment; a CDR-grafted antibody; a single-chain antibody; a single chain antibody fragment; modified antibody, a bispecific antibody, a chimeric antibody; a diabody; a triabody; a tetrabody; a minibody; a linear antibody; a chelating recombinant antibody; a bibody; a tribody; an intrabody; a nanobody; a small modular immunopharmaceutical (SMIP); an antigen-binding-domain immunoglobulin fusion protein; a camelid antibody; a shark single domain antibody, an avian antibody, a VHH-containing antibody; a F(ab); a F(ab)′; F(ab)′ 2 ; scFv; an Fc fragment generated from the heavy chain constant region of an antibody; a reduced IgG fragment; an Fc fusion protein; a domain antibody; a VL; a VNAR; a VH; and a VHH.
18 . The recombinant gram-negative Pseudomonas fluorescens host cell of claim 16 , wherein the antibody, antibody fragment, or derivative thereof, binds to a target selected from: a cytokine; a chemokine; a drug; a cell-surface protein; a growth factor; a growth factor receptor; immune checkpoint molecule, and a blood factor.
19 . The recombinant gram-negative Pseudomonas fluorescens host cell of claim 16 , wherein the antibody, antibody fragment, or derivative thereof is a Fab′.
20 . The recombinant gram-negative Pseudomonas fluorescens host cell of claim 19 , wherein the Fab′ binds to a target selected from: Carcinoembryonic antigen (CEA); CD22; fibrin II, beta chain; TNF-alpha; and NCA-90 (granulocyte antigen).
21 . The recombinant gram-negative Pseudomonas fluorescens host cell of claim 16 , wherein the at least one expression construct encoding the antibody, antibody fragment, or derivative thereof comprises at least one nucleic acid sequence encoding a heavy chain, at least one nucleic acid sequence encoding a light chain, or both, wherein the heavy chain is full-length or a heavy chain fragment, and the light chain is full-length or a light chain fragment.
22 . The recombinant gram-negative Pseudomonas fluorescens host cell of claim 21 , wherein each heavy chain-encoding nucleic acid sequence and each light chain-encoding nucleic acid sequence is individually operably linked to an independently selected nucleic acid sequence encoding a periplasmic secretion signal.
23 . The recombinant gram-negative Pseudomonas fluorescens host cell of claim 20 , wherein the Fab′ is certolizumab.
24 . The recombinant gram-negative Pseudomonas fluorescens host cell of claim 1 , wherein the host cell is deficient in:
(i) the first protease activity;
(ii) the second protease activity;
(iii) the activity of a serralysin precursor having the amino acid sequence set forth as SEQ ID NO: 9, or a serralysin precursor related protein having at least 60% similarity or at least 60% identity to the amino acid sequence set forth as SEQ ID NO: 9;
(iv) an Hs1U protease having the amino acid sequence set forth as SEQ ID NO: 37, or an Hs1U related protein having at least 60% similarity or at least 60% identity to the amino acid sequence set forth as SEQ ID NO: 37; and
(v) an Hs1V protease having the amino acid sequence set forth as SEQ ID NO: 38, or an Hs1V related protein having at least 60% similarity or at least 60% identity to the amino acid sequence set forth as SEQ ID NO: 38.
25 . The recombinant gram-negative Pseudomonas fluorescens host cell of claim 24 , wherein the host cell overexpresses an exogenous disulfide isomerase having at least 60% similarity or at least 60% identity to an amino acid sequence set forth as SEQ ID NO: 27.
26 . The recombinant gram-negative Pseudomonas fluorescens host cell of claim 1 , wherein the cell is:
(i) lsc::lacIQ1;
(ii) Prc1-
(iii) Prc2-
(iii) Hs1U-
(iv) Hs1V-
(v) MepM1-
(vi) PyrF-
and (vii) deficient in a serralysin precursor that is: a serralysin precursor having the amino acid sequence set forth as SEQ ID NO: 9; or a serralysin precursor related protein having at least 60% similarity or at least 60% identity to the amino acid sequence set forth as SEQ ID NO: 9; wherein the serralysin precursor deficiency results from a mutation in a gene encoding the serralysin precursor.
27 . The recombinant gram-negative Pseudomonas fluorescens host cell of claim 26 , further comprising an expression vector comprising a nucleic acid sequence encoding DegP2 S219A as set forth in SEQ ID NO: 29 or disulfide isomerase PDIA6 as set forth in SEQ ID NO: 27.
28 . The recombinant gram-negative Pseudomonas fluorescens host cell of claim 1 , further comprising an expression vector encoding a recombinant protein.
29 . The recombinant gram-negative Pseudomonas fluorescens host cell of claim 27 , wherein the expression vector encodes a Fab′.
30 . The recombinant gram-negative Pseudomonas fluorescens host cell of claim 1 , wherein the recombinant gram-negative Pseudomonas fluorescens host cell has the genotype of strain; STR94976.
31 . The recombinant gram-negative Pseudomonas fluorescens bacterial host cell of claim 30 , further comprising an expression construct-STR94976, for use in producing a recombinant anti-TNF-alpha Fab′.
32 . A method for producing a recombinant protein of interest comprising: (a) recovering the recombinant protein of interest from the recombinant gram-negative Pseudomonas fluorescens host cell of claim 1 , wherein the recombinant gram-negative Pseudomonas fluorescens host cell is transformed with a plasmid comprising a nucleic acid encoding the recombinant protein of interest.
33 . The method of claim 32 , wherein transcription of the nucleic acid sequence encoding the recombinant protein of interest is regulated by an inducible promoter.
34 . The method of claim 32 , wherein the recombinant gram-negative Pseudomonas fluorescens host cell can grow to high cell density.
35 . The method of claim 32 , wherein the fermentation conditions comprise induction of the inducible promoter at: an OD575 of about 80 to about 160, a culture pH of about 5.8 to about 7.0, a temperature of about 28-33 deg C., fed-batch, and a titer range of about 0.2 to about 5 g/L.
36 . The method of claim 32 , wherein the recombinant gram-negative Pseudomonas fluorescens host cell grows to a cell density that is increased in comparison to a control cell grown under the same fermentation conditions.
37 . The method of claim 36 , wherein the increase in cell density is about 2-fold to about 15-fold.
38 . The method of claim 32 , further comprising: (b) measuring the yield of intact, soluble, and/or active, recombinant protein of interest recovered from the recombinant gram-negative bacterial host cell, wherein the measured yield of intact, soluble, and/or active, recombinant protein is about 0.1 to about 10 g/L.
39 . The method of claim 38 , further comprising: (c) measuring the yield of recombinant protein of interest recovered from a control cell that is intact, soluble, active, or a combination thereof.
40 . The method of claim 39 , further comprising (d) comparing the yield measured in step (b) to the yield measured in step (c), wherein the yield measured in step (b) is about 2-fold to about 100-fold higher than that measured in step (c).
41 . A recombinant gram-negative Pseudomonas fluorescens host cell, wherein the host cell is:
(a) deficient in a first protease activity, wherein the deficient first protease activity results from mutations in
i. a gene encoding a Prc1 tail-specific protease having the amino acid sequence set forth as SEQ ID NO: 33; and,
ii. a gene encoding a Prc2 tail-specific protease having the amino acid sequence set forth as SEQ ID NO: 35; and
(b) deficient in a second protease activity, wherein the deficient second protease activity results from a mutation in a gene encoding a MepM1 murein DD-endopeptidase having the amino acid sequence set forth as SEQ ID NO: 1; and additionally wherein the host cell is further:
(c) deficient in one additional protease activity, wherein the deficient additional protease activity results from a mutation of a gene encoding a serralysin precursor having the amino acid sequence set forth as SEQ ID NO: 9;
(d) deficient in one or more autolytic factor activity, wherein the deficient autolytic factor activity results from a mutation in at least one gene encoding an autolytic factor independently selected from: a linear gramicidin synthase subunit D, or a hemolysin precursor;
(e) overexpresses a mutant periplasmic serine endoprotease;
(f) overexpresses a disulfide isomerase folding modulator-having at least 60% similarity or at least 60% identity to an amino acid sequence set forth as SEQ ID NO: 27; or
(g) any combination of (c), (d), (e) and (f).
42 . The recombinant gram-negative Pseudomonas fluorescens host cell of claim 41 , wherein the host is (a) and (b).
43 . The recombinant gram-negative Pseudomonas fluorescens host cell of claim 41 , wherein the host cell is (a), (b), and (e), wherein the mutant serine peptidase of (e) is as set forth in SEQ ID NO: 29.
44 . The recombinant gram-negative Pseudomonas fluorescens host cell of claim 41 , wherein the host cell is (a), (b), (e), and (f), wherein the mutant serine peptidase of (e) is as set forth in SEQ ID NO: 29.