Protoporphyrinogen oxidase (PPO) resistance plant
Disclosed are transgenic plant expressing a fungal protoporphyrinogen oxidase (PPO) enzyme and methods for conferring Herbicide Tolerance (HT) to PPO-type inhibitor herbicides, and controlling weeds, by selectively protecting the transgenic crop expressing the fungal PPO enzyme.
1 . A transgenic plant comprising a nucleic acid encoding an exogenous fungal-derived Protoporphyrinogen Oxidase (PPO) enzyme conferring resistance to at least one PPO-inhibitor herbicide, wherein the exogenous fungal-derived PPO enzyme comprises an amino acid sequence having at least 95% similarity to the amino acid sequence of any one of: SEQ ID NOs: 1-7, SEQ ID NO: 86, SEQ ID NOs: 88-89, or SEQ ID NOs: 91-99.
2 . The transgenic plant of claim 1 , wherein the fungal-derived PPO enzyme comprises an amino acid sequence having at least 98% identity to the one amino acid sequence of any one of SEQ ID NOs: 1-7, SEQ ID NO: 86, SEQ ID NOs: 88-89, or SEQ ID NOs: 91-99.
3 . The transgenic plant of claim 1 , wherein the fungal-derived PPO enzyme further comprises a plant chloroplast transit peptide.
4 . The transgenic plant of claim 1 , wherein the fungal-derived PPO is from a fungal species selected from Aspergillus sp., Fusarium sp., Rhizopus sp., Penicillium sp., Trichoderma sp., Rhodotorula sp., Hesseltinella sp., Spizellomyces sp. Rhizophagus sp., Alternariav sp., and Cladosporium sp.
5 . The transgenic plant of claim 4 , wherein the fungal-derived PPO enzyme is from a fungal species selected from the group consisting of Aspergillus sp., Fusarium sp., Rhizopus sp., Penicillium sp., and Trichoderma sp.
6 . The transgenic plant of claim 4 , wherein the fungal-derived PPO is from a fungal species selected from Aspergillus sp., Penicillium sp., Trichoderma sp., Rhodotorula sp., and Hesseltinella sp.
7 . The transgenic plant of claim 1 , wherein the fungal-derived PPO enzyme is from a fungal species of the clade leotiomyceta.
8 . The transgenic plant of claim 1 , wherein the plant is selected from the group consisting of maize, wheat, rice, barley, soybean, cowpea, chickpea, cotton, sorghum, beans, rapeseed/canola, alfalfa, flax, sunflower, safflower, millet, rye, sugarcane, sugar beet, cocoa, tea, Brassica , coffee, sweet potato, flax, peanut, clover, lettuce, tomato, cucurbits, cassava, potato, carrot, radish, pea, lentils, cabbage, cauliflower, broccoli, brussels sprouts, peppers, pineapple, citrus, apple, pear, peach, apricot, walnut, avocado, banana, palm, eucalyptus , poplar, pine, coconut, orchids, petunia , carnations, roses, switchgrass, prairie grasses, indian grasses, big bluestem grass, and camelina.
9 . The transgenic plant of claim 8 , wherein the plant is selected from the group consisting of maize, wheat, rice, soybean, cowpea, chickpea, cotton, sorghum, rapeseed/canola, alfalfa, sunflower, sugarcane, sugar beet and camelina.
10 . The transgenic plant of claim 1 , wherein the at least one PPO inhibitor is selected from the group consisting of herbicide families Phenylpyrazoles, Pyrimidindiones, Oxazolidinedione, Diphenylethers, Oxadiazoles, N-phenylphthalimides, Triazinone, Thiadiazoles, Triazolinones, and Triazolopyridinones.
11 . The transgenic plant of claim 1 , wherein the plant is a broad leaf plant.
12 . The transgenic plant of claim 1 , wherein the plant is a cereal plant.
13 . A seed of the transgenic plant of claim 1 , wherein the seed comprises said nucleic acid.