Polynucleotides, primers, and methods for detection of transgenic event, genetic construct, kit for detection material from a plant sample, event CTC91087-6, insect-resistant sugarcane plant, and method for producing an insect-resistant sugarcane plant, plant cell, plant part or seed
The present invention relates to the field of biotechnology. More precisely, a genetic construct and method for producing a transgenic plant event, especially a sugarcane event { Saccharum spp.), which is resistant to infestation by the Diatraea saccharalis pest, popularly known as a pest, ordinary borer, reed borer or just borer is described. The invention describes the event, the methods for event identification as well as the insertion detection method based on the unique region of intersection between the insert and the host genome and the flanking regions that characterize it.
1 . A recombinant nucleic acid molecule comprising the polynucleotide sequence of SEQ ID NO: 5.
2 . The recombinant nucleic acid molecule of claim 1 , comprising the polynucleotide sequence of SEQ ID NO: 22.
3 . A genetically modified sugarcane plant comprising a recombinant nucleic acid molecule comprising the polynucleotide sequence of SEQ ID NO: 5, wherein the plant is insect-resistant.
4 . A method of producing a genetically modified sugarcane plant that has improved insect resistance, comprising introducing a transfer DNA (T-DNA) fragment having the polynucleotide sequence of SEQ ID NO: 2 at a specific locus of genome positioned between sequences selected from the group consisting of SEQ ID NOs: 23 and 24, wherein a genetically modified sugarcane plant comprising a recombinant nucleic acid molecule comprising the polynucleotide sequence of SEQ ID NO: 5 is obtained.
5 . The genetically modified sugarcane plant according to claim 3 , comprising a recombinant nucleic acid molecule comprising the polynucleotide sequence of SEQ ID NO: 22.
6 . The method according to claim 4 , wherein the genetically modified sugarcane plant comprises a recombinant nucleic acid molecule comprising the polynucleotide sequence of SEQ ID NO: 22.