IP Library › Granted Patent US 12674212
Granted Patent B2
US 12674212 · App. 17/712,012 · Granted Jul 7, 2026

PCR method for the detection of equine rotavirus group B (ERVB) in biological samples

Inventors: Feng Li (Lexington, KY); Dan Wang (Lexington, KY); Erdal Erol (Lexington, KY); Tirth Uprety (Lexington, KY)
Assignee: University of Kentucky Research Foundation
C12Q1/701C12Q1/686C12N2720/12321
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Quick Facts
Patent No.
US 12674212
App. No.
17/712,012
Granted
Jul 7, 2026
Kind
B2
Abstract

Provided herein are a set of primers and probes for detecting rotavirus. The set of primers and probes includes at least one forward primer selected from the group including SEQ ID NOS: 17, 20, 23, 26, 29, 32, 35, 38, 41, 44, 47, 50, 53, 56, 59, 62, 65, 68, 71, 74, 77, 80, 83, 86, 89, 92, 95, 98, 101, 104, 107, 110, 113, 116, 119, 122, 125, 128, 131, 134, 137, 140, 143, 146, 149, 152, 155, 158, 161, 164, 167, 204, 207, 210, and combinations thereof; at least one reverse primer selected from the group including SEQ ID NOS: 18, 21, 24, 27, 30, 33, 36, 39, 42, 45, 48, 51, 54, 57, 60, 63, 66, 69, 72, 75, 78, 81, 84, 87, 90, 93, 96, 99, 102, 105, 108, 111, 114, 117, 120, 123, 126, 129, 132, 135, 138, 141, 144, 147, 150, 153, 156, 159, 162, 165, 168, 205, 208, 211, and combinations thereof; and at least one probe selected from the group including SEQ ID NOS: 19, 22, 25, 28, 31, 34, 37, 40, 43, 46, 49, 52, 55, 58, 61, 64, 67, 70, 73, 76, 79, 82, 85, 88, 91, 94, 97, 100, 103, 106, 109, 112, 115, 118, 121, 124, 127, 130, 133, 136, 139, 142, 145, 148, 151, 154, 157, 160, 163, 166, 169, 206, 209, 212, and combinations thereof. Also provided herein is a method for detecting rotavirus using the set of primers and probes.

Claims (7)

1 . A method for detecting equine rotavirus group B (ERVB) in an equine biological sample, the method comprising:

extracting viral ribonucleic acid (RNA) from the sample;

performing polymerase chain reaction (PCR) analysis on a VP7 segment of ERVB using a primer and probe set comprising SEQ ID NOs: 210, 211, and 212; and

determining whether the sample contains ERVB based on signal generated during amplification.

2 . The method of claim 1 , wherein the equine biological sample is a fecal sample.

3 . The method of claim 1 , wherein said primer and probe set exhibits no amplification of porcine or bovine RVB VP7 sequences under assay conditions.

4 . The method of claim 1 , wherein the polymerase chain reaction (PCR) analysis is a real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR).