Biochip and detection method
A biochip is capable of highly sensitively detecting a measurement target substance. The biochip includes a selective-binding substance capable of selectively binding to the measurement target substance, the selective-binding substance being immobilized on a surface of a substrate through a polymer containing a unit represented by Formula (Ia) or (Ib): wherein, R 1 represents C 1 -C 4 alkylene; R 2 represents R 3 , OR 4 , or NHR 5 ; R 3 and R 5 each independently represent a hydrogen atom or C 1 -C 4 alkyl; R 4 represents C 1 -C 4 alkyl; R 6 represents C 1 -C 2 alkylene; and R 7 and R 8 each independently represent a hydrogen atom or C 1 -C 4 alkyl.
1 . A biochip comprising a selective-binding substance capable of selectively binding to a measurement target substance, the selective-binding substance being immobilized on a surface of a substrate through a polymer containing a unit represented by Formula (Ia) or (Ib):
wherein, in Formulae (Ia) and (Ib),
R 1 represents C 1 -C 4 alkylene; R 2 represents R 3 , OR 4 , or NHR 5 ; R 3 represents a hydrogen atom or C 1 -C 4 alkyl; R 4 represents C 1 -C 4 alkyl; R 5 represents C 1 -C 4 alkyl; R 6 represents C 1 -C 2 alkylene; and R 7 and R 8 each independently represent a hydrogen atom or C 1 -C 4 alkyl.
2 . The biochip according to claim 1 , wherein the polymer is represented by the Formula (Ia).
3 . The biochip according to claim 1 , wherein the polymer is a heteropolymer.
4 . The biochip according to claim 3 , wherein the heteropolymer is a heteropolymer comprising a unit represented by Formula (II):
wherein R 1 represents C 1 -C 4 alkylene.
5 . The biochip according to claim 1 , wherein the polymer has a number average molecular weight of 300 g/mol to 1,000,000 g/mol.
6 . The biochip according to claim 1 , wherein the selective-binding substance is a nucleic acid or a protein.
7 . A method of detecting a measurement target substance using the biochip according to claim 1 , the biochip comprising a selective-binding substance capable of selectively binding to the measurement target substance, the selective-binding substance being immobilized on a surface of a substrate, the method comprising:
bringing a specimen containing the measurement target substance into contact with the surface of the substrate, to thereby form a complex with the selective-binding substance; and
detecting the complex.
8 . A biochip comprising a selective-binding substance capable of selectively binding to a measurement target substance, the selective-binding substance being immobilized on a surface of a substrate through a polymer containing a unit represented by (Ib):
wherein, in (Ib),
R 1 represents C 1 -C 4 alkylene; R 2 represents R 3 , OR 4 , or NHR 5 ; R 3 and R 5 each independently represents a hydrogen atom or C 1 -C 4 alkyl; R 4 represents C 1 -C 4 alkyl; R 6 represents C 1 -C 2 alkylene; and R 7 and R 8 each independently represent a hydrogen atom or C 1 -C 4 alkyl.