Egg replacement food product and method of producing thereof comprising microbial protein biomass
Disclosed is a method of producing an egg replacement food product that includes mixing a protein microbial biomass powder with water to obtain a wet mixture, hydrating the wet mixture at room temperature, mixing the wet mixture at high speed, hydrating the mixed wet mixture at 6 C degrees, mixing the hydrated wet mixture with an oil at high speed and emulsifying the mixed wet mixture, homogenizing the emulsified mixed wet mixture, heating the homogenized wet mixture up to from 85 C up to 90 C degrees, and adding sodium chloride (NaCl) and citric acid to the heated wet mixture and mixing to obtain pH from 3.5 up to 4.0.
1 . A method of producing an egg replacement food product, the method comprising:
mixing a protein microbial biomass powder, comprising isolated bacterial strain deposited as VTT-E-193585 or a derivative thereof with water to obtain a wet mixture;
hydrating the wet mixture at room temperature;
mixing the wet mixture at high speed;
hydrating the mixed wet mixture at 6 C degrees;
mixing the hydrated wet mixture with an oil at high speed and emulsifying the mixed wet mixture;
homogenizing the emulsified mixed wet mixture;
heating the homogenized wet mixture up to from 85 C up to 90 C degrees;
adding sodium chloride (NaCl) and citric acid to the heated wet mixture and mixing to obtain pH
from 3.5 up to 4.0.
2 . The method according to claim 1 further comprising heating the mixed wet mixture having pH from 3.5 up to 4.0.
3 . The method according to claim 1 , wherein total weight of the wet mixture with a pH from 3.5 up to 4.0 comprises:
from 76% up to 78% of water;
from 9% up to 10% of the protein microbial biomass powder comprising isolated bacterial strain deposited as VTT-E-193585 or a derivative thereof;
from 0.7% up to 1.1% of sodium chloride (NaCl);
from 9% up to 10% of the oil.
4 . The method according to claim 1 , wherein the oil is selected to be at least one of a rapeseed oil, a sunflower oil, a soy oil.
5 . The method according to claim 1 , wherein homogenizing is carried out at from 500 bars up to 1200 bars.
6 . The method according to claim 1 , wherein the protein microbial biomass powder is produced via upstream and downstream processes, the downstream process comprising following steps:
cultivating bacterial cells by gas fermentation to obtain a microbial biomass;
separating a liquid phase and a solid phase of the microbial biomass and concentrating the microbial biomass by removing the liquid phase;
drying the microbial biomass to obtain the protein microbial biomass powder.
7 . The method according to claim 6 , wherein the downstream process further comprises incubating the microbial biomass with a heat treatment at temperature from 55 C up to
75 C degrees from 15 minutes up to 40 minutes.
8 . The method according to claim 6 , wherein the downstream process further comprises homogenizing the bacterial cells of the microbial biomass before drying step.
9 . The method according to claim 8 , wherein the homogenizing is carried out at pressure from 800 bars up to 2000 bars for at least one run.
10 . The method according to claim 6 , wherein a feed for cultivating by gas fermentation comprises at least one of selected from CO2, CH4, H2, O2, NH3, at least one mineral.
11 . The method according to claim 6 further comprising adjusting the microbial biomass pH to be from 7.4 up to 8.0 after separating the liquid phase.
12 . An egg replacement food product wherein total weight of the egg replacement food product comprises
from 76% up to 78% of water;
from 9% up to 10% of a protein microbial biomass powder comprising isolated bacterial strain deposited as VTT-E-193585 or a derivative thereof;
from 0.7% up to 1.1% of sodium chloride (NaCl);
from 9% up to 10% of an oil.
13 . The egg replacement food product according to claim 12 , wherein the oil is selected to be at least one of a rapeseed oil, a sunflower oil, a soy oil.