Gold nanoparticle intranasal vaccine compositions
Provided herein, inter alia, are complexes comprising nanoparticles attached to viral proteins or nucleic acids encoding said viral proteins. Methods for making and using said complexes are provided. Compositions including the complexes are contemplated to be useful for treating and/or preventing viral infections.
1 . An intranasal vaccine composition comprising complexes of:
(a) a nanoparticle consisting of a gold core coated with an outer layer consisting of a uniform monolayer of a cationic polysaccharide, wherein the cationic polysaccharide is chitosan and
the outer layer is covalently attached to the gold core; and
(b) a nucleic acid encoding a SARS-CoV-2 S protein, wherein the nucleic acid is attached to said nanoparticle and
(c) a pharmaceutically acceptable excipient,
wherein the complexes are from about 20 nm to about 80 nm in diameter.
2 . The complex of claim 1 , comprising a plurality of nucleic acids encoding said pulmonary viral protein or fragment thereof.
3 . A method of treating or preventing a pulmonary viral disease in a subject, the method comprising intranasal administration of the pharmaceutical composition of claim 1 to the subject.
4 . A method of immunizing a subject susceptible to a pulmonary viral disease, the method comprising intranasal administration of the pharmaceutical composition of claim 1 to the subject.