Method of manufacturing a tissue regeneration patch

1 . A method of manufacturing a tissue regeneration patch, comprising:

A) preparing a micronized adipose tissue extract;

B) injecting the micronized adipose tissue extract into a mold of a predetermined shape;

C) cooling the mold into which the adipose tissue extract is injected at a temperature of more than −20° C. and less than −10° C.; and

D) removing the mold to obtain a tissue regeneration patch,

wherein step A) comprises the step of pulverizing an adipose tissue in stages sequentially using a1) a first filter having a pore diameter of 2 mm to 5 mm, a second filter having a pore diameter of 400 μm to 800 μm, and a third filter having a pore diameter of 150 μm to 250 μm.

2 . The method according to claim 1 , wherein further comprising the step of a2) mixing the adipose tissue micronized by the third filter with physiological saline and leaving the mixture to stand, and after phase separation into an aqueous phase layer and an organic phase layer, removing the aqueous phase layer.

3 . The method according to claim 1 , wherein in step a1), the adipose tissue is pulverized by repeatedly passing the adipose tissue back and forth through each filter.

4 . The method according to claim 1 , wherein the adipose tissue is autologous adipose tissue.

5 . The method according to claim 1 , wherein the mold of the predetermined shape is a 2D or 3D shape corresponding to an area of damaged or lost tissue.

6 . The method according to claim 1 , wherein the step C) is performed on a cooling plate at a temperature of more than −20° C. and less than −10° C.

7 . The method according to claim 6 , wherein the mold into which the adipose tissue extract is injected is positioned on a polyurethane film provided on the cooling plate.

8 . The method according to claim 1 , wherein the steps B) to D) are performed in a bioprinter equipped with a cooling plate under a sterile atmosphere.

9 . The method according to claim 1 , wherein the thickness of the tissue regeneration patch is at least 1 mm.

10 . The method according to claim 1 , wherein the tissue regeneration patch is for treating damaged or lost skin tissue.