Chimeric peptide-modified SIS membrane, preparation method therefor and application thereof

Inventors: Pengfei Wei (Beijing, CN); Huasheng Wang (Beijing, CN); Bo Zhao (Beijing, CN)

Assignee: Beijing Biosis Healing Biological Technology Co., Ltd.

A61L27/3629 A61L27/227 A61L27/54 C07K14/001

View Patent ↗

Loading inventors, assignments & file history…

Monitor This Case

Get email alerts when status or documents change.

Order Certified Copies

Most orders are placed with the USPTO same day — all within 24 business hours.

Order via The Patent Place →

Pre-filled with this patent's details

Quick Facts

Patent No.: US 12678540
App. No.: 18/557,084
Granted: Jul 14, 2026
Kind: B2

External Links

USPTO Patent Center↗ Full Patent PDF↗

Abstract

A chimeric peptide-modified SIS membrane, a preparation method therefor and an application thereof. The chimeric peptide-modified SIS membrane is used to promote the expression of ITG-α3, ITG-β1, BMP2, RUNX2, ALP and OPN, and inhibits the growth of Streptococcus gordonii and Streptococcus sanguinis , thereby enabling the SIS membrane to exert antibacterial, osteogenic and healing-promoting biological functions. The SIS membrane (that is, a chimeric peptide-modified GBR film) can be used for clinical treatment of infectious bone defects.

Claims (34)

1 . A chimeric peptide-modified SIS membrane, wherein the sequence of the chimeric peptide comprises at least one sequence in a group consisting of sequences shown as follows:

(i) a group consisting of a sequence as set forth in SEQ ID NO: 9, a sequence as set forth in SEQ ID NO: 10, a sequence as set forth in SEQ ID NO: 11, and a sequence as set forth in SEQ ID NO: 12; and

(ii) a sequence with a conservative substitution as compared to the sequence set forth in (i).

2 . The chimeric peptide-modified SIS membrane according to claim 1 , wherein the sequence of the chimeric peptide consists of at least one sequence in a group consisting of the following sequences: (i) a group consisting of a sequence as set forth in SEQ ID NO: 9, a sequence as set forth in SEQ ID NO: 10, a sequence as set forth in SEQ ID NO: 11, and a sequence as set forth in SEQ ID NO: 12; and (ii) a sequence with a conservative substitution as compared to the sequence set forth in (i).

3 . The chimeric peptide-modified SIS membrane according to claim 1 , wherein a method for the modification comprises the following steps:

(a) dissolving the chimeric peptide into a solvent to obtain a solution containing the chimeric peptide;

(b) applying the solution obtained in step (a) to the surface of the SIS membrane; and

4 . The chimeric peptide-modified SIS membrane according to claim 1 , wherein a method for preparing the SIS membrane comprises the following steps:

(i) subjecting a small intestinal submucosa material to primary treatment; and

(ii) subjecting the small intestinal submucosa material obtained in step (i) to immunogen removal treatment.

5 . The chimeric peptide-modified SIS membrane according to claim 4 , wherein the method for preparing the SIS membrane further comprises the following steps:

(iii) laminating the small intestinal submucosa material obtained in step (ii); and

(iv) subjecting the laminated small intestinal submucosa material to drying treatment.

6 . A method for preparing a chimeric peptide-modified SIS membrane, wherein the method comprises the following steps:

(a) dissolving the chimeric peptide into a solvent to obtain a solution containing the chimeric peptide;

(b) applying the solution obtained in step (a) to the surface of the SIS membrane; and

(c) drying the SIS membrane with the solution on the surface thereof to obtain the chimeric peptide-modified SIS membrane;

wherein the sequence of the chimeric peptide comprises or consists of at least one in a group consisting of sequences shown as follow:

(ii) a sequence with a conservative substitution as compared to the sequence set forth in (i).

7 . The preparation method for the chimeric peptide-modified SIS membrane according to claim 6 , wherein a method for preparing the SIS membrane comprises the following steps:

(i) subjecting a small intestinal submucosa material to primary treatment; and

(ii) subjecting the small intestinal submucosa material obtained in step (i) to immunogen removal treatment.

8 . The preparation method for the chimeric peptide-modified SIS membrane according to claim 7 , wherein the method for preparing the SIS membrane further comprises the following steps:

(iii) laminating the small intestinal submucosa material obtained in step (ii); and

(iv) subjecting the laminated small intestinal submucosa material to drying treatment.

9 . A method for preparing an antibacterial biomaterial, wherein the method comprises utilizing the chimeric peptide-modified SIS membrane according to claim 1 .

10 . A method for treating an infectious bone defect, wherein the method comprises a step of administering, to a subject, the chimeric peptide-modified SIS membrane according to claim 1 .

11 . The chimeric peptide-modified SIS membrane according to claim 3 , wherein in step (b), soaking the SIS membrane in the solution obtained in step (a).

12 . The chimeric peptide-modified SIS membrane according to claim 6 , wherein in step (b), soaking the SIS membrane in the solution obtained in step (a).

13 . A method for preparing an osteogenic biomaterial, wherein the method comprises utilizing the chimeric peptide-modified SIS membrane according to claim 1 .

14 . A method for preparing a healing-promoting biomaterial, wherein the method comprises utilizing the chimeric peptide-modified SIS membrane according to claim 1 .

15 . A method for preparing a biomaterial for treating an infectious bone defect, wherein the method comprises utilizing the chimeric peptide-modified SIS membrane according to claim 1 .