BioActive implant for reconstruction of bone defect, deformity, and NonUnion
A bioactive orthopedic implant is provided defined as a rod with a surface and a treated surface area for increased surface area, a freeze-dried hydrophilic hydrogel network physically cross-linked via charged polymers and salt-ions onto the treated surface area, biologies trapped and thereby hosted within the hydrogel network, and covalently reactive macromonomers chemically cross-linked within the hydrogel network to strengthen the hydrogel network itself and to the rod. The surface area of the bioactive orthopedic implant can be coated with covalently linkable molecules which are chemical cross-linked with the covalently reactive macromonomers to increase adhesion of the chemically and physically cross-linked hydrophilic hydrogel network to the rod. The rod can be an interconnected porous rod and where the biologies is hosted with pores of the interconnected porous rod. The bioactive orthopedic implant can be sized for implantation in between two bone segments, a bone tunnel, or a fracture.
1 . A method of reconstructing a bone defect, deformity or nonunion, comprising:
(a) providing a freeze-dried bioactive orthopedic implant, wherein the freeze-dried bioactive orthopedic implant comprises:
(i) a scaffold comprising a treated surface area,
(ii) a hydrophilic hydrogel network, comprising:
a) a charged polymer physically cross-linked to the scaffold,
b) covalently reactive macromonomers chemically cross-linked to the scaffold, and
(iii) one or more biologic agents within the hydrophilic hydrogel network; and
(b) implanting the freeze-dried bioactive orthopedic implant into a patient in need thereof, wherein the implant provides sustained-release of the one or more biologic agents.
2 . The method of claim 1 , wherein the scaffold is a rod.
3 . The method of claim 1 , wherein the scaffold is porous.
4 . The method of claim 1 , wherein the scaffold comprises polycaprolactone-beta-tricalcium phosphate (PCL-TCP).
5 . The method of claim 1 , wherein the surface of the scaffold is treated with an acid or a base.
6 . The method of claim 1 , wherein the surface of the scaffold is treated with a base.
7 . The method of claim 1 , wherein the hydrophilic hydrogel network comprises polymerized aminopropyl methacrylamide (APMA), gelatin methacrylate (GelMA), polyethylene glycol dimethacrylate (PEGDMA), or a combination thereof.
8 . The method of claim 1 , wherein the surface of the scaffold is treated with a salt.
9 . The method of claim 8 , wherein the salt comprises a calcium salt, magnesium salt, strontium salt, zinc salt, aluminum salt, titanium salt or a combination thereof.
10 . The method of claim 8 , wherein the salt comprises a calcium salt.
11 . The method of claim 1 , wherein the charged polymer comprises alginate.
12 . The method of claim 1 , wherein the charged polymer is physically crosslinked to the scaffold via an ionic interaction.
13 . The method of claim 12 , wherein the ionic interaction is between a calcium salt on the surface of the scaffold and the charged polymer.
14 . The method of claim 13 , wherein the calcium salt comprises CaSO 4 , CaCl 2 or a combination thereof.
15 . The method of claim 1 , wherein the covalently reactive macromonomers comprise gelatin methacrylate (GelMA) and polyethylene glycol dimethacrylate (PEGDMA).
16 . The method of claim 1 , wherein the biologic agents comprise a growth factor.
17 . The method of claim 16 , wherein the growth factor comprises bone morphogenetic proteins (BMP).