Cartridge and testing device
The present invention relates to the field of biomedical technology and discloses a cartridge and a testing device. The cartridge comprises a sample lysis compartment, a first sample mixing compartment and a first PCR compartment; a first valve is disposed between the sample lysis compartment and the first sample mixing compartment, the first valve controls the flowing or blocking of the sample between the sample lysis compartment and the first sample mixing compartment; a fourth valve is disposed between the first PCR compartment and the first sample mixing compartment, the fourth valve controls the flow or blocking of the sample between the first sample mixing compartment and the first PCR compartment; a first reagent is provided in the first sample mixing compartment. In the compartment, nucleic acids in the sample mix with the first reagent and is then sent to the first PCR compartment for amplification.
1 . A cartridge comprising a sample lysis compartment ( 130 ), a first sample mixing compartment ( 151 ) and a first PCR compartment ( 161 );
wherein a first valve is provided between the sample lysis compartment ( 130 ) and the first sample mixing compartment ( 151 ), and the first valve is configured to control the flow or blocking of a sample between the sample lysis compartment ( 130 ) and the first sample mixing compartment ( 151 ); a fourth valve is provided between the first PCR compartment ( 161 ) and the first sample mixing compartment ( 151 ), and the fourth valve is configured to control the flow or blocking of the sample between the first sample mixing compartment ( 151 ) and the first PCR compartment ( 161 ); and the first sample mixing compartment ( 151 ) is provided with a first reagent configured to mix with nucleic acids in the sample;
wherein the cartridge further comprises a pneumatic module, the pneumatic module is configured to drive sample flow between the sample lysis compartment ( 130 ), the first sample mixing compartment ( 151 ), and the first PCR compartment ( 161 );
wherein the pneumatic module comprises a third air hole ( 203 ) and a ninth air hole ( 209 ), the third air hole ( 203 ) communicates with the first sample mixing compartment ( 151 ); the first PCR compartment ( 161 ) communicates with the ninth air hole ( 209 ); and a first waterproof and breathable film ( 1510 ) is provided on the ninth air hole ( 209 ), the first waterproof and breathable film ( 1510 ) is configured for discharging air from the first PCR compartment ( 161 ) while blocking liquid from flowing out of the first waterproof and breathable film ( 1510 ); and
wherein the third air hole ( 203 ) communicates with a second air pump to draw or push air contained in the first sample mixing compartment ( 151 ).
2 . The cartridge according to claim 1 , further comprising a sample filtration compartment ( 140 );
wherein the sample filtration compartment ( 140 ) communicates with the first sample mixing compartment ( 151 ); and
the first valve is located between the sample lysis compartment ( 130 ) and the sample filtration compartment ( 140 ).
3 . The cartridge according to claim 1 , further comprising a first damping compartment ( 1011 ) communicating with the first PCR compartment ( 161 ).
4 . The cartridge according to claim 1 , further comprising a sample addition compartment ( 110 ) communicating with the sample lysis compartment ( 130 ).
5 . The cartridge according to claim 1 , wherein the first sample mixing compartment ( 151 ) is further provided with a first mixing bead ( 710 ), the first mixing bead ( 710 ) is configured to mix the sample and the first reagent thoroughly by its movement.
6 . The cartridge according to claim 1 , further comprising a cassette sample addition nozzle ( 300 ), wherein the cassette sample addition nozzle ( 300 ) communicates with the sample addition compartment ( 110 ), and is configured used for adding the sample into the sample addition compartment ( 110 ).
7 . The cartridge according to claim 1 , wherein a plate ( 131 ) is provided in the sample lysis compartment ( 130 ), the plate ( 131 ) is configured to prevent the sample from splashing when entering the sample lysis compartment ( 130 ) and configured to balance a force acting upon the cartridge.
8 . The cartridge according to claim 1 , wherein the first valve is disposed below the sample lysis compartment ( 130 ) and communicates with a bottom of the sample lysis compartment ( 130 ).
9 . The cartridge according to claim 1 , wherein
the cartridge further comprises a sixth valve, the ninth air hole ( 209 ) communicates with the first PCR compartment ( 161 ) through the sixth valve in a switchable manner; and
the pneumatic module further comprises a sixth air hole ( 206 ), the sixth air hole ( 206 ) communicates with the first sample mixing compartment ( 151 ), the sixth air hole ( 206 ) is provided with a second waterproof and breathable film ( 1520 ), when the first sample mixing compartment ( 151 ) is filled up, the second waterproof and breathable film ( 1520 ) is configured to prevent liquid from flowing out of the second waterproof and breathable film ( 1520 ).
10 . The cartridge according to claim 1 , wherein the pneumatic module further comprises a first air hole ( 201 ), and a first air channel ( 210 ) is provided at the cartridge;
said first air hole ( 201 ) communicates with the first air channel ( 210 ), the first air channel ( 210 ) communicates with the sample lysis compartment ( 130 ); and
said first air hole ( 201 ) is configured to communicate with a first air pump to draw or push air contained in the sample lysis compartment ( 130 ).
11 . The cartridge according to claim 9 , further comprising a fifth air hole ( 205 ) and a second air compartment ( 920 ),
wherein the fifth air hole ( 205 ) and the sixth air hole ( 206 ) are both located in the second air compartment ( 920 ), the second air compartment ( 920 ) is provided with a sealing film, and the sealing film is located on an outer side of the second waterproof and breathable film ( 1520 ); and
wherein the fifth air hole ( 205 ) communicates with the third air hole ( 203 ), the fifth air hole ( 205 ) and the sixth air hole ( 206 ) exchange air in the second air compartment ( 920 ).
12 . A testing device, comprising the cartridge according to claim 1 and a supporting instrument;
the supporting instrument comprises at least the following modules:
a first driving module configured for driving the opening and closing of the first valve;
a fourth driving module configured for driving the opening and closing of the fourth valve;
a first magnetic module ( 2300 ) configured for providing magnetic force;
a heating module ( 2200 ) configured for heating a sample mixture;
a first temperature control module configured for providing a heating and cooling cycle;
a first optical detection module configured for assessing amplification in a chamber.
13 . The testing device according to claim 12 , the device further comprises a rack ( 2000 ),
the first driving module, the fourth driving module, the first magnetic module ( 2300 ), the heating module ( 2200 ), and the first temperature control module are all disposed on the rack ( 2000 );
an installation slot ( 2100 ) is provided on the rack ( 2000 ) at a position corresponding to the positions of the first driving module, the fourth driving module, the first magnetic module ( 2300 ), the heating module ( 2200 ), and the first temperature control module; the cartridge is vertically inserted into the installation slot ( 2100 ).
14 . A testing device, comprising the cartridge according to claim 2 and a supporting instrument;
the supporting instrument comprises at least the following modules:
a first driving module configured for driving the opening and closing of the first valve;
a fourth driving module configured for driving the opening and closing of the fourth valve;
a first magnetic module ( 2300 ) configured for providing magnetic force;
a heating module ( 2200 ) configured for heating a sample mixture;
a first temperature control module configured for providing a heating and cooling cycle;
a first optical detection module configured for assessing amplification in a chamber.
15 . A testing device, comprising the cartridge according to claim 3 and a supporting instrument;
the supporting instrument comprises at least the following modules:
a first driving module configured for driving the opening and closing of the first valve;
a fourth driving module configured for driving the opening and closing of the fourth valve;
a first magnetic module ( 2300 ) configured for providing magnetic force;
a heating module ( 2200 ) configured for heating a sample mixture;
a first temperature control module configured for providing a heating and cooling cycle;
a first optical detection module configured for assessing amplification in a chamber.
16 . A testing device, comprising the cartridge according to claim 4 and a supporting instrument;
the supporting instrument comprises at least the following modules:
a first driving module configured for driving the opening and closing of the first valve;
a fourth driving module configured for driving the opening and closing of the fourth valve;
a first magnetic module ( 2300 ) configured for providing magnetic force;
a heating module ( 2200 ) configured for heating a sample mixture;
a first temperature control module configured for providing a heating and cooling cycle;
a first optical detection module configured for assessing amplification in a chamber.
17 . A testing device, comprising the cartridge according to claim 5 and a supporting instrument;
the supporting instrument comprises at least the following modules:
a first driving module configured for driving the opening and closing of the first valve;
a fourth driving module configured for driving the opening and closing of the fourth valve;
a first magnetic module ( 2300 ) configured for providing magnetic force;
a heating module ( 2200 ) configured for heating a sample mixture;
a first temperature control module configured for providing a heating and cooling cycle;
a first optical detection module configured for assessing amplification in a chamber.
18 . A testing device, comprising the cartridge according to claim 6 and a supporting instrument;
the supporting instrument comprises at least the following modules:
a first driving module configured for driving the opening and closing of the first valve;
a fourth driving module configured for driving the opening and closing of the fourth valve;
a first magnetic module ( 2300 ) configured for providing magnetic force;
a heating module ( 2200 ) configured for heating a sample mixture;
a first temperature control module configured for providing a heating and cooling cycle;
a first optical detection module configured for assessing amplification in a chamber.
19 . A testing device, comprising the cartridge according to claim 7 and a supporting instrument;
the supporting instrument comprises at least the following modules:
a first driving module configured for driving the opening and closing of the first valve;
a fourth driving module configured for driving the opening and closing of the fourth valve;
a first magnetic module ( 2300 ) configured for providing magnetic force;
a heating module ( 2200 ) configured for heating a sample mixture;
a first temperature control module configured for providing a heating and cooling cycle;
a first optical detection module configured for assessing amplification in a chamber.
20 . The cartridge according to claim 4 , wherein the cartridge further comprises a siphon tube ( 120 ), the sample addition compartment ( 110 ) and the sample lysis compartment ( 130 ) communicate with each other through the siphon tube ( 120 ).
21 . The cartridge according to claim 20 , wherein a first choke valve ( 801 ) is provided in the siphon tube ( 120 ); the first choke valve ( 801 ) is configured to control the flow or blocking of the sample and/or air between the sample addition compartment ( 110 ) and the sample lysis compartment ( 130 ).
22 . The cartridge according to claim 1 , wherein the first reagent is a first lyophilized bead ( 610 ).
23 . The cartridge according to claim 22 , wherein the first lyophilized bead ( 610 ) comprises an excipient, the weight of the excipient accounts for 20%-60% of the total weight of the first lyophilized bead ( 610 ).
24 . The cartridge according to claim 6 , wherein the cartridge further comprises a lid ( 400 ), the lid ( 400 ) is configured to cap the cassette sample addition nozzle ( 300 ); and
the lid ( 400 ) is provided with a step ( 401 ), the step ( 401 ) and a through-hole ( 301 ) of the cassette sample addition nozzle ( 300 ) are in an interference fit.
25 . The cartridge according to claim 10 , wherein the pneumatic module further comprises a second air hole ( 202 ) and a first air compartment ( 910 );
said first air hole ( 201 ) and the second air hole ( 202 ) are located in the first air compartment ( 910 ), the first air hole ( 201 ) communicates with the first air channel ( 210 ) through the second air hole ( 202 ); and
said first waterproof and breathable film ( 1510 ) is sealed over the first air hole ( 201 ) and the second air hole ( 202 ); a sealing film is provided at an outer side of the first waterproof and breathable film ( 1510 ), so that the first air hole ( 201 ) and the second air hole ( 202 ) exchange air within the first air compartment ( 910 ).
26 . The cartridge according to claim 11 , wherein the pneumatic module further comprises a fourth air hole ( 204 ), a second air channel ( 220 ) and a third air channel ( 230 );
one end of the third air hole ( 203 ) communicates with the second air pump, one end of the second air channel ( 220 ) communicates with the other end of the third air hole ( 203 ); the second air channel ( 220 ) communicates with the third air channel ( 230 ) through the fourth air hole ( 204 ); the third air channel ( 230 ) communicates with the fifth air hole ( 205 ); and
the third air channel ( 230 ) and one end of the third air hole ( 203 ) that communicates with the second air pump are located at one surface of the cartridge, the second air channel ( 220 ) and the third air channel ( 230 ) are located at a surface opposite to the cartridge.