Purification process based on magnetic beads
There is provided a process for the separation of molecules from a suspension comprising cells at a concentration of at least 56×10 6 cells/ml, comprising the steps of: a) providing magnetic particles having a specific interaction with said molecules to be separated, b) mixing the magnetic particles with the cell suspension containing the molecules, c) bringing the cell suspension in contact with a magnetic field provided by a magnetic separation device to collect the magnetic particles, d) decreasing or removing said magnetic field and collecting said magnetic particles carrying said molecules, and e) removing said molecules from said magnetic particles, to provide a concentrated fraction of said molecules, and/or provide partial or complete removal of impurities and cells from the fraction containing the molecules. Advantages include that the yield increases, the volume of the bioreactor and other equipment can be made smaller so that the process becomes more economical.
1 . A process for the separation of molecules from a suspension comprising cells at a concentration of at least 56×10 6 cells/ml, comprising the steps of:
growing the cells in a cell medium in a bioreactor, wherein a perfusion method is used, wherein spent cell medium depleted of nutrients and including cell waste products is removed and wherein fresh cell medium is provided to the cells in the bioreactor at the same rate as spent cell medium is removed, wherein molecules with a molecular weight over a determined cut off value are not removed together with the spent cell medium, and wherein the cells in the cell medium form the suspension comprising the cells, which suspension is used in the following steps without any filtration;
providing magnetic particles having a specific interaction with said molecules to be separated;
mixing the magnetic particles with the suspension containing the molecules;
bringing the suspension in contact with a magnetic field provided by a magnetic separation device to collect the magnetic particles;
decreasing or removing said magnetic field and collecting said magnetic particles carrying said molecules; and
removing said molecules from said magnetic particles, to provide a concentrated fraction of said molecules, and/or provide partial or complete removal of impurities and cells from the fraction containing the molecules.
2 . The process according to claim 1 , wherein a bead capacity usage is at least 60%.
3 . The process according to claim 1 , wherein the magnetic particles and the suspension containing the molecules are in contact no more than 45 minutes after the mixing.
4 . The process according to claim 1 , wherein the suspension comprising cells has a concentration of at least 70×10 6 cells/ml.
5 . The process according to claim 1 , wherein the suspension comprising cells is such that the volume of the cells occupies at least 12% of the culture volume and is any one from the group consisting of cell culture, microorganism fermentation and cell suspension derived from cell tissue.
6 . The process according to claim 1 , wherein the magnetic particles comprise a component selected from the group consisting of agarose, silica, cellulose, polyvinyl alcohol, polyethylene glycol, polystyrene, acrylate, dextran, and derivatives thereof.
7 . The process according to claim 1 , wherein the magnetic particles comprise at least one functional group selected from the group consisting of —SH, —SS-pyridine, —COOH, —NH2, —CHO, —OH, phenol, anhydride, epoxy, an S—Au, an amide, an aminoethyl group, a diethylaminoethyl group, a quaternary ammonium group, a carboxymethyl group, a phosphate group, a sulfopropyl group, IDA (iminodiacetic acid) and derivatives thereof, TED (tris (carboxymethyl) ethylenediamine) and derivatives thereof, CM-Asp (carboxymethylated aspartic acid) and its derivatives, NTA (nitrilotriacetic acid) and its derivatives, TREN (tris(2-aminoethyl)amine) and its derivatives, DPA (lutidine) and its derivatives, C6-S gel (hexylthio group) and its derivatives, and EDTA (ethylenediaminetetraacetic acid) and its derivatives.
8 . The process according to claim 1 , wherein the magnetic particles carry at least one selected from the group consisting of C n H m (1≤n≤20, 4≤m≤42), phenol and its derivatives, thiophenol and a group of derivatives of thiophenol, and a group consisting of mercaptopyridine and its derivatives.
9 . The process according to claim 1 , wherein the magnetic particles comprise at least one functional group comprising at least one group which is produced by reacting with at least one compound selected from the group consisting of divinyl sulfone, benzene anthracene, imidazole, periodate, trichloro-S-triazine, toluenesulfonate, diazo compound, isourea salt, carbodiimide, hydrazine, epichlorohydrin, glutaraldehyde, cyanogen bromide, double Ethylene oxide, carbonyl diimidazole, N-hydroxysuccinimide, silane, and derivatives thereof.
10 . The process according to claim 1 wherein the affinity is obtained using molecules suitable for molecular interaction introduced on magnetic particles.
11 . The process of claim 10 , wherein the molecule suitable for molecular interaction is at least one selected from the group consisting of organic molecules, proteins, antigens, enzymes, enzyme inhibitors, cofactors, hormones, toxins, vitamins, glycoconjugates, nucleic acids, antibodies, peptides, lectins, and carbohydrates.
12 . The process according to claim 1 , wherein the magnetic particles comprise particles of at least one magnetic material embedded in a polymer matrix, and wherein the polymer matrix comprises functional groups.
13 . The process according to claim 1 , wherein the suspension containing the molecules comprises at least one selected from the group consisting of alive cells, dead cells, ruptured cells, lysed cells, cell debris, cell membrane, proteins, peptides, DNA, RNA, ions, amino acids, organic compounds, salts, water, solvents and/or metals.
14 . The process of claim 13 , wherein the cells are eukaryotic cells.
15 . The process of claim 14 , wherein the eukaryotic cells are selected from the group consisting of mammalian cells, human cells, avian cells, insect cells and plant cells.
16 . The process of claim 15 , wherein the cells are selected from the group consisting of: CHO, CHO-DBX11, CHO-DG44, CHO-S, CHO-K1, Vero, BHK, HeLa, COS, MDCK, HEK-293, HEK-293T, HEK-293S, HEK-293F, L293, NIH-3T3, W138, BT483, Hs578T, HTB2, BT20, T47D, NSO, CRL7030, HsS78Bst cells, PER.C6, SP2, SPO, hybridoma, MRC-5, MDCK, WI-98, CAP, EB66, AGE1.CR, CR, Trichoplusia ni, Spodoptera Frugiperda, SF9, SF21, Hi5, mesenchymal stem cells, induced pluripotent stem cells, embryonic stem cells, primary cells, Nicotiana tabacum, BY2, Nicotiana benthamiana, Oriza sativa, Arabidopis thaliana, and Daucus carota.
17 . The process according to claim 1 , wherein the molecules are polypetides, proteins, antibodies, enzymes, viral vectors, viruses, polysaccharides, polymers, exosomes, mRNA, siRNA, or any combination of these, or assemblies derived from these, or assemblies derived from these and associated with a small molecule of a size less than 1000 kDa.
18 . The process of claim 1 , wherein the cells are selected from the group consisting of Escherichia coli, yeast, Saccharomyces cerevisiae, Pichia pastoris, and Aspergillus niger.
19 . The process according to claim 1 , wherein the cells are ruptured or lysed by mechanical disruption, ultrasonication, osmotic shock, freeze-thaw, pressure homogenisation, heat treatment or chemical action.
20 . The process according to claim 1 , wherein the volume of the suspension is at least 100 ml.
21 . The process according to claim 1 , wherein said magnetic particles are washed at least once before removing said molecules from said magnetic particles.