Composition and methods to treat ectodermal dysplasia 2, Clouston type
The present technology relates generally to compositions and methods of preventing or treating diseases associated with mutant Cx26, Cx32, and/or Cx30hemichannels. The present technology also relates to administering the anti-Cx26 hemichannel antibodies in effective amounts to treat a subject suffering from, or predisposed to, ectodermal dysplasia 2, Clouston type (OMIM No. 129500), Keratitis-ichthyosis-deafness syndrome (KIDS; OMIM No. 148210), and/or type X Charcot-Marie-Tooth neuropathy (CMTXI; OMIM No. 302800).
1 . A method for treating ectodermal dysplasia 2, Clouston type (OMIM No. 129500) in a subject in need thereof, comprising administering to the subject an effective amount of an antibody or antigen binding fragment thereof comprising a heavy chain immunoglobulin variable domain (V H ) and a light chain immunoglobulin variable domain (V L ),
wherein the V H comprises complementarity determining regions V H CDR1, V H CDR2 and V H CDR3, wherein the V H CDR1 comprises an amino acid sequence set forth in SEQ ID NO: 3, V H CDR2 comprises an amino acid sequence set forth in SEQ ID NO: 4, and V H CDR3 comprises an amino acid sequence set forth in SEQ ID NO: 5; and
wherein the VL comprises complementarity determining regions V L CDR1, V L CDR2 and V L CDR3, wherein the V L CDR1 comprises an amino acid sequence set forth in SEQ ID NO: 6, V L CDR2 comprises an amino acid sequence set forth in SEQ ID NO: 7, and V L CDR3 comprises an amino acid sequence set forth in SEQ ID NO: 8.
2 . The method of claim 1 , wherein the method alleviates one or more symptoms of ectodermal dysplasia 2, Clouston type (OMIM No. 129500) selected from the group consisting of dystrophy of the nails, hypoplasticity and deformation of nails, and increased susceptibility to paronychial infections.
3 . The method of claim 1 , wherein the V H comprises an amino acid sequence set forth in SEQ ID NO: 11.
4 . The method of claim 1 , wherein the V L comprises an amino acid sequence set forth in SEQ ID NO: 12.
5 . The method of claim 1 , wherein the antibody or antigen binding fragment thereof is administered by a route selected from the group consisting of parenteral, oral, inhalation, topical, intraocular, iontophoretic, and transmucosal administration.
6 . The method of claim 1 , wherein the antibody or antigen binding fragment thereof is administered by a parenteral route.
7 . The method of claim 1 , wherein the antibody or antigen binding fragment thereof is administered by a topical route.
8 . The method of claim 1 , wherein the antibody or antigen binding fragment thereof is an antibody, scFv, (scFv) 2 , scFv-Fc, Fab, Fab′, F(ab′) 2 or an scFv-Fc antibody.
9 . The method of claim 1 , wherein the antibody or antigen binding fragment thereof is an scFv-Fc antibody.
10 . The method of claim 1 , wherein the antibody or antigen binding fragment thereof is formulated as an ointment, salve, gel, or cream.
11 . The method of claim 1 , wherein the antibody or antigen binding fragment thereof is formulated as an injectable.